文献类型：期刊文献

英文题名：Astragaloside IV Attenuates Trinitrobenzene Sulphonic Acid (TNBS)-Induced Colitis via Improving Mucosal Barrier Function: Role of Goblet Cells

作者：Zang, Kai-hong[1,2];Qin, Hong-yan[3];Duan, Hai-Jing[1,2];Ma, Qing-lin[1];Ren, Yuan[1,2]

第一作者：Zang, Kai-hong;臧凯宏

通信作者：Ren, Y[1]

机构：[1]Gansu Univ Tradit Chinese Med, Coll Pharm, Dingxi East Rd, Lanzhou 730000, Gansu, Peoples R China;[2]Gansu Key Lab Pharmacol & Toxicol Tradit Chinese, Dingxi East Rd, Lanzhou 730000, Gansu, Peoples R China;[3]Lanzhou Univ, Hosp 1, Dept Pharm, Lanzhou 730000, Gansu, Peoples R China

第一机构：甘肃中医药大学药学院（西北中藏药协同创新中心办公室）

通信机构：[1]corresponding author), Gansu Univ Tradit Chinese Med, Coll Pharm, Dingxi East Rd, Lanzhou 730000, Gansu, Peoples R China.|[1073501e14fb35863569f]甘肃中医药大学药学院（西北中藏药协同创新中心办公室）;[10735]甘肃中医药大学;

年份：2018

卷号：14

期号：8

起止页码：1160

外文期刊名：INTERNATIONAL JOURNAL OF PHARMACOLOGY

收录：;Scopus(收录号：2-s2.0-85055467693);WOS:【SCI-EXPANDED(收录号:WOS:000452552500014)】；

基金：This study was supported by the National Science Foundation of China (81860728, 81341145, 81400596), the Fundamental Research Funds for the Central Universities (lzujbky-2014-223), Natural Science Foundation of gansu province (No 17JR5RA162).

语种：英文

外文关键词：Astragaloside IV; colitis; mucosal barrier function; goblet cells; differentiation

摘要：Background and Objective: Astragaloside IV, the main bioactive ingredient of Radix Astragali showed anti-inflammation and would healing effect in practice. This study aimed to investigate the therapeutic effect of astragaloside IV (AS-IV) on experimental colitis as well as its role in mucosal healing and barrier function. Materials and Methods: TNBS-induced colitis rats were orally treated with AS-IV at the dose of 10,20 and 40 mg kg(-1) per day for 8 consecutive days. After drug treatment, histological damage score and myeloperoxidase activity of the colon tissue were detected, mucosal barrier function was evaluated by measuring the serum level of D-lactate and diamine oxidase and colonic goblet cells and the mRNA expression of Mucin were also evaluated by immuno-histochemistry and RT-PCR, respectively. The proteins and genes in Wnt and Notch signaling were further investigated by Western blot and RT-PCR to identify the effect of AS-IV on the differentiation of goblet cells. Results: Histological scores, myeloperoxidase activity and serum level of D-lactate and diamine oxidase in colitis rats were significantly increased, while mRNA expression of Muc-2 and Muc-3 were significantly decreased. AS-IV administration significantly reduced histological scores, myeloperoxidase activity and the level of D-lactate and diamine oxidase in colitis rats and the expression of Muc-2 and Muc-3 were markedly increased. Moreover, the protein expression in Wnt signaling, i.e., Lrp5, Lrp6 and beta-catenin, in the colon of colitis rats was significantly elevated, but the genes expression in Notch signaling, i.e., Rath1, Gfi1 and Klf4, in colitis rats were markedly decreased, and these alteration of Wnt and Notch signaling in colitis rats were markedly reversed by AS-IV administration. Conclusion: AS-IVattenuates colon inflammation in colitis rats via improving mucosal barrier function, the regulatory effect on the proliferation and differentiation of goblet cells may contribute to the therapeutic role of AS-IV in colitis.