文献类型：期刊文献

英文题名：ZIF-8 gated dual miRNA-sensing nanoplatform for enzyme-free amplification and precision diagnosis of metabolic dysfunction-associated steatohepatitis

作者：Zhao, Jing[1];Xu, Leqi[1];Zhao, Tingting[2,3];Zhang, Huali[1];Zhang, Xia[3];Di, Duolong[2];Hai, Jun[2];Pei, Dong[2,3]

第一作者：赵静

通信作者：Zhao, TT[1];Hai, J[1];Pei, D[1];Zhao, TT[2];Pei, D[2]

机构：[1]Gansu Univ Chinese Med, Coll Pharm, Lanzhou 730000, Peoples R China;[2]Lanzhou Inst Chem Phys, Chinese Acad Sci CAS, CAS Key Lab Chem Northwestern Plant Resources, Key Lab Nat Med Gansu Prov, Lanzhou 730000, Peoples R China;[3]Qingdao Ctr Resource Chem & New Mat, Qingdao 266000, Shandong, Peoples R China

第一机构：甘肃中医药大学药学院（西北中藏药协同创新中心办公室）

通信机构：[1]corresponding author), Lanzhou Inst Chem Phys, Chinese Acad Sci CAS, CAS Key Lab Chem Northwestern Plant Resources, Key Lab Nat Med Gansu Prov, Lanzhou 730000, Peoples R China;[2]corresponding author), Qingdao Ctr Resource Chem & New Mat, Qingdao 266000, Shandong, Peoples R China.

年份：2026

卷号：300

外文期刊名：TALANTA

收录：;EI(收录号：20255019703722);Scopus(收录号：2-s2.0-105024481132);WOS:【SCI-EXPANDED(收录号:WOS:001640917200001)】；

基金：This work was financed by Science and Technology program of Gansu Province (24JRRA049), Science Foundation of Shandong Province of China (ZR2024QB044), Key R&D Program of Gansu Province (25YFFA033), Gansu Science Fund for Basic Creative Research Groups (25JRRA469),Yunnan Province Major Scientific and Technological Project (202302AE090007 & 202402AA310034).

语种：英文

外文关键词：Dual miRNA sensing; Accurate imaging; Metabolic dysfunction-associated steatohepati-; tis; Enzyme-free signal amplification method; Metal-organic framework

摘要：Ultrasensitive and reliable imaging of miRNAs in living cells facilitates advanced understanding of diseases and pathological progression. However, synchronous detection of multicomponent miRNAs remains challenging due to obstacles in the efficient co-delivery of probes and their inherently low abundance. Herein, we present a versatile Hairpins@ZIF-8 nanoplatform by integrating ZIF-8 with three hairpins for dual miRNA sensing. This system enables synchronous cellular transfection of nucleic acid probes, acid-responsive degradation preventing nonspecific activation during endocytosis-dependent delivery, and probe stabilization with degradation-released metal ions serving as cofactors for enzyme-free signal amplification. The ZIF-8 gated dual miRNA-sensing nanoplatform demonstrated high sensitivity in vitro, synchronously detecting miRNA-122 and miRNA-21 with detection limits of 52 pM and 48 pM, respectively. Benefiting from its superior performance, it was applied to metabolic dysfunction-associated steatohepatitis (MASH) models and visualized time-dependent dual-miRNA expression changes in sodium palmitate-induced L02 cells, showing promise for early MASH diagnosis and pathological elucidation. Notably, we provide the first visual evidence demonstrating that miRNA-122 regulates miRNA-21 expression. This strategy provides both a tool for deciphering MASH progression and a platform for advancing miRNA diagnostics and therapeutics.