文献类型：期刊文献

中文题名：高脂血症大鼠hepcidin的表达异常及川芎嗪的干预研究

英文题名：Study on Abnormal Expression of Hepcidin in Hyperlipidemia Rats and Effects of Tetramethylpyrazine on the Expression of Hepcidin

作者：孙明月[1];郭春雨[1];王景尚[1];刘欣[1];张淼[1];王琳[2];殷惠军[1,2]

第一作者：孙明月

机构：[1]中国中医科学院西苑医院,北京100091;[2]甘肃中医学院,兰州730000

第一机构：中国中医科学院西苑医院,北京100091

年份：2015

卷号：44

期号：8

起止页码：17

中文期刊名：医学研究杂志

外文期刊名：Journal of Medical Research

收录：CSTPCD

基金：国家自然科学基金资助项目(81102845);2013年甘肃高校"飞天学者特聘计划"项目

语种：中文

中文关键词：铁调素;川芎嗪;血脂代谢异常

外文关键词：Hepcidin;Tetramethylpyrazine; Hyperlipemia

摘要：目的探讨高脂血症大鼠铁调素(hepcidin)表达的变化及川芎嗪对其的干预效应。方法 40只SD大鼠随机分为空白对照组、模型组、肝素组及川芎嗪组4组,每组10只。除空白对照组以普通饲料喂养外,其余3组均以高脂饲料喂养。8周后,空白对照组及模型组给予生理盐水2ml/(kg·d),肝素组和川芎嗪组分别给予肝素钠5mg/(kg·d)、盐酸川芎嗪注射液40mg/(kg·d)腹腔注射,连续7天。腹主动脉取血后分离血清,生化法检测各组血脂,ELISA法检测血清铁调素(hepcidin)、白细胞介素1(IL-1)、白细胞介素6(IL-6)及肿瘤坏死因子-α(TNF-α)的表达。结果与空白对照组相比,模型组、肝素组及川芎嗪组血脂均显著升高(P<0.05);与空白对照组相比,模型组血清hepcidin、IL-1、IL-6及TNF-α含量均显著升高(P<0.05);与模型组相比,肝素组和川芎嗪组血清hepcidin、IL-1、IL-6及TNF-α含量均显著降低(P<0.05),但肝素组素组下降更为明显。结论随着血脂升高,炎性因子及hepcidin呈现高表达状态,应用川芎嗪后hepcidin水平下降,炎性因子表达也下降,说明川芎嗪具有抑制血清hepcidin高表达的作用,这可能与其对炎性因子的抑制有关。
Objective To observe the expression changes of hepcidin in hyperlipidemia rats and the effects of tetramethylpyrazine （TMP） on the expression of hepeidin. Methods Forty SD rats were randomly divided into 4 groups： blank control group,model group, heparin group and TMP group. Except the blank control group, all other groups were fed with high - fat diet for 8 weeks. The blank control group and model group were injection with normal saline at 2ml/（ kg d）. Rats in the TMP group were injected with TMP at 40mg/（ kg ~ d） , heparin at 5mg/（ kg ~ d） was injected to those in the heparin treated group. After rats were given medicine for 7 days, the level of blood lipid, serum hepcidin, IL - 1 ,IL - 6 and TNF - ct were detected. Results As compared with blank control group, the level of hep- cidin, IL - 1, IL - 6 and TNF - c~ in serum was increased significantly in model group （ P 〈 0.05 ）. Compared with the model group, the level of hepcidin, IL - 1, IL - 6 and TNF - ct in serum was obviously decreased in TMP group and heparin group （ P 〈 O. 05 ）. Conclusion Inflammatory factors and hepcidin expression was increased with improved blood lipid levels. After rats were given TMP, the level of se- rum hepcidin and Inflammatory factors were decreased. It is suggested that TMP showed obvious inhibition on high expression of hepcidin, and its inhibitory effect might be correlated to inhibiting Inflammatory factors.