文献类型：期刊文献

英文题名：A Novel Peptide-Based Fluorometric and Colorimetric Probe for Cascade Detection of Copper(Ii) and Glyphosate: Applications in Real Sample, Cells Imaging, Test Strips and Smartphone-Assisted Recognition

作者：Wang, Peng[1,3]; Zheng, Maoyue[1]; Zhou, Miao[1]; Wang, Yiwen[1]; Shang, Shiyu[1]; He, Qian[1]; An, Yong[2]

第一作者：Wang, Peng

机构：[1] Chemical Synthesis and Pollution Control Key Laboratory of Sichuan Province, College of Chemistry and Chemical Engineering, China West Normal University, Shida Road 1#, Nanchong, 637009, China; [2] The First School of Clinical Medicine, Gansu University of Chinese Medicine, Gansu, Lanzhou, 730030, China; [3] Sichuan Engineering Research Center for Molecular Targeted Diagnostic & Therapeutic Drugs, Department of Chemistry, Xihua University, PR, Chengdu, 610039, China

第一机构：Chemical Synthesis and Pollution Control Key Laboratory of Sichuan Province, College of Chemistry and Chemical Engineering, China West Normal University, Shida Road 1#, Nanchong, 637009, China

年份：2024

外文期刊名：SSRN

收录：EI(收录号：20240458415)

语种：英文

外文关键词：Colorimetric analysis - Fluorescence imaging - Quantum yield - Thermography (imaging) - Titration

摘要：A new dual-signals peptide-based probe (FAHF) by conjugating 5-carboxy fluorescein (5-FAM) fluorophore with a tripeptide backbone (Ala-His-Phe-NH2) was successfully synthesized. According to design, FAHF exhibited favorable properties for sensing Cu2+ by fluorescence quenching and colorimetric color change among various metal ions, such as good water solubility, excellent selectivity, wide pH range and high quantum yield. In addition, the binding constant of FAHF-Cu2+ was 1.2 × 1010 M-2 and the detection limit (LOD) of Cu2+ was attained 51.1 nM. The fluorescence titration, Job’s plot and high resolution mass spectrometry (ESI-HRMS) analysis indicated that a 2:1 complex formation between FAHF and Cu2+. Notably, the resulting FAHF-Cu2+ complex displayed "turn-on" fluorescence and colorimetric response upon addition of glyphosate based on displacement approach, and could realize naked eye recognition with color changed under 365 nm UV light and visible light. FAHF-Cu2+ complex exhibited a good linear relationship at ultra-low concentrations glyphosate with a detection limit of 46.8 nM. Furthermore, fluorescence imaging applications demonstrated that FAHF was successfully applied for cascade detection of Cu2+ and glyphosate in living Hacat cells based on low toxicity. Moreover, FAHF was successfully used to determine Cu2+ and glyphosate in two water samples with satisfactory results. More importantly, the test strips with FAHF was fabricated for identifying Cu2+ and glyphosate under 365 nm UV light and visible light, and the RGB channel in smartphone was used to convert different solution colors into digital values, which was successfully used for semi-quantitative recognition of Cu2+ and glyphosate. ? 2024, The Authors. All rights reserved.