详细信息
文献类型:期刊文献
中文题名:木贼中三种黄酮苷的含量测定
英文题名:Determination of three flavonoids in Equisetum hiemale by TLCS
作者:赵磊[1];李冲[2];张承忠[2];杨锡[1]
第一作者:赵磊
机构:[1]甘肃中医学院,兰州730000;[2]兰州大学,兰州730000
第一机构:甘肃中医药大学
年份:2005
卷号:22
期号:2
起止页码:159
中文期刊名:中国现代应用药学
外文期刊名:Chinese Journal of Modern Applied Pharmacy
收录:CSTPCD;;北大核心:【北大核心2004】;CSCD:【CSCD_E2011_2012】;
语种:中文
中文关键词:木贼;异槲皮素;山柰酚-3-芸香糖-7-葡萄糖苷;山柰酚-3,7-双葡萄糖苷;黄酮类化合物;薄层扫描法
外文关键词:Equisetum hiemale L. Isoquercetin; kaempferol-3 - rutinoside-7-glucoside; kaempferol-3,7-diglucoside, flavonoids; TLCS
摘要:目的测定木贼中三种黄酮苷的含量.方法采用双波长薄层扫描法测定木贼中三种黄酮苷含量.结果异槲皮素,山柰酚-3-芸香糖-7-葡萄糖苷,山柰酚-3,7-双葡萄糖苷分别在0.5~2.5μg,0.2~1.0μg,0.2~1.0μg范围内呈良好的线性关系,平均回收率分别为97.96%,98.06%,97.43%,RSD分别为2.08%,2.31%,2.46%.结论用薄层扫描法测定木贼中三种黄酮苷的含量是可行的,为木贼的合理利用和质量控制提供了一定的科学依据.
OBJECTIVE To establish the method for de termination of three flavonoids in Equisetum hiemale L.METHOD TLC was applied.RESULTS Isoquercetin, kaempferol-3-rutinoside-7-glucoside, kaempferol- 3,7- diglucoside showed a good linear relationship respectively at a rang of 0.5~2.5μg,0.2~1.0μg, 0.2~1.0μg. The average recovery was 97.96% ,98.06%,97.43% and RSD was 2.08%,2.31%,2.46% respectively.CONCLUSION It is reproducible and practical to determine the co ntent of three flavonoids in Equisetum hiemale L. by the TLCS method. The study provides referential information for quality control, usage and exploitation of this medicinal plant.
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