文献类型：期刊文献

中文题名：槟榔多酚提取物中总多酚和儿茶素类的含量测定

英文题名：Determination of total polyphenols and catechins in betel nut polyphenol extract

作者：马江红[1,2];王荣[2];杜兴[3];赵安鹏[2];王子晗[1,2];张浩波[1]

第一作者：马江红

机构：[1]甘肃中医药大学药学院,甘肃兰州730000;[2]解放军联勤保障部队第九四〇医院药剂科,甘肃兰州730050;[3]甘肃省药品检验研究院,甘肃兰州730070

第一机构：甘肃中医药大学药学院（西北中藏药协同创新中心办公室）

年份：2022

卷号：40

期号：3

起止页码：243

中文期刊名：药学实践杂志

外文期刊名：Journal of Pharmaceutical Practice

收录：CSTPCD

基金：国家自然科学基金项目(81673508);2020年中央高校创新团队项目(3192020009)。

语种：中文

中文关键词：槟榔总多酚;儿茶素类;高效液相色谱法;紫外分光光度法

外文关键词：betelnut total polyphenols;catechins;HPLC;UV spectrophotometry

摘要：目的建立槟榔多酚提取物中总多酚和儿茶素类含量的测定方法,为槟榔多酚提取物的质量控制提供参考。方法采用紫外分光光度法对槟榔提取物中总多酚的含量进行测定;高效液相色谱法对儿茶素类物质进行含量测定。结果紫外分光光度法测定槟榔提取物中总多酚含量在9.8~58.8μg/ml范围内线性关系良好,平均加样回收率为98.44%,RSD为1.4%;在高效液相色谱测定中,儿茶素、表儿茶素和原儿茶酸3种主要成分完全分离,在各自范围内线性关系良好,平均加样回收率为99.17%~101.67%,RSD为1.2%~2.5%。结论建立的含量测定方法简单、稳定可靠,可用于槟榔多酚提取物的定量分析,为槟榔多酚提取物的质量控制提供实验依据。
Objective To establish a method for the determination of total polyphenols and catechins in betel nut polyphenols extract,and provide reference for the quality control of betel nut polyphenols extract.Methods The content of total phenol in betel nut extract was determined by ultraviolet spectrophotometry.The content of catechins was determined by HPLC.Results The linear range of total polyphenols in betel nuts extract was 9.8~58.8μg/ml.The three components of catechin,epicatechin and protocatechuic acid were completely separated by HPLC,and the linear relationship was good in their respective ranges,with the recoveries between 99.17%and 101.67%,the RSD between 1.2%and 2.5%.Conclusion The established method is simple,stable and reliable,which could be used for the quantitative analysis of betel polyphenol extract,and provide experimental basis for the quality control of betel polyphenol extract.