文献类型：期刊文献

中文题名：黄芪提取工艺优选及其药物配比优化研究

英文题名：Study on the Optimization of Extraction Technology and Drug Ratio of Huangqi(Astragali Radix)

作者：高飞云[1,2];刘婷[1,2];宋沁洁[1,2];李东辉[1,2];吴红伟[1,2];罗慧英[1];李越峰[1,2,3]

第一作者：高飞云

机构：[1]甘肃中医药大学,甘肃兰州730000;[2]甘肃省中药质量与标准研究重点实验室,甘肃兰州730000;[3]甘肃省中药制药工艺工程研究中心,甘肃兰州730000

第一机构：甘肃中医药大学

年份：2023

卷号：25

期号：3

起止页码：53

中文期刊名：辽宁中医药大学学报

外文期刊名：Journal of Liaoning University of Traditional Chinese Medicine

收录：CSTPCD

基金：国家自然科学基金项目(81960713);甘肃省自然科学基金创新基地和人才计划项目(18JR3RA197);甘肃省中药质量与标准研究重点实验室开放基金项目(ZYZL18-008);甘肃省中药制药工艺工程研究中心开放课题项目(ZYGY202003);国家药品监督管理局中药材及饮片质量控制重点实验室项目(2020GSMPA-KL15)。

语种：中文

中文关键词：黄芪;提取工艺;Box-Behnken响应面法;配比;优化工艺

外文关键词：Huangqi(Astragali Radix);extraction process;Box-Behnken response surface method;proportion ratio;optimization of process

摘要：目的采用正交试验法优选黄芪水提液提取的最佳条件,利用Box-Behnken响应面法优化黄芪及药物配比研究。方法水浴回流法提取黄芪,以干膏率为评价指标,在料液比、提取时间、提取次数3个单因素的基础上进行正交试验优选黄芪水提液的最佳提取条件。该实验以黄芪为原料,在单因素的基础上,以黄芪浓缩液/药物混合液、辅料1加入量、辅料2加入量、柠檬酸加入量为考察因素,优化黄芪及药物最佳配比。结果黄芪水提液最佳提取条件为料液比1∶6,提取时间1 h,提取次数3次,黄芪的提取率为44.75%。黄芪及药物配比为以50 g药物混合液为基准,黄芪浓缩液/药物混合液为29%,辅料1加入量0.15 g,辅料2加入量20 g,柠檬酸加入量0.2 g,最终感官评分达92.5分,与预测值相近,说明模型拟合良好,该优化工艺准确可行。结论黄芪最佳提取工艺及其药物配比优化结果重现性好,工艺简便易行,可以为黄芪系列产品的开发奠定基础,有一定的开发应用前景。
Objective To use the orthogonal test method,the best conditions for extracting the extraction extraction extraction of Huangqi(Astragali Radix),using the Box-Behnken response surface method to optimize the study of Huangqi(Astragali Radix)and drug ratio.Methods The water bath retraction method extracts Huangqi(Astragali Radix),and the orthogonal test is preferably the optimal extraction conditions for the orthogonal test by three single factors based on the liquid ratio,extraction time,and the number of extracted times.Similarly,in this experiment,Huangqi(Astragali Radix)was used as raw material,on the basis of single factor,Huangqi(Astragali Radix)concentrate/drug mixture,excipient 1,excipient 2 and citric acid were used as factors to optimize the ratio of Huangqi(Astragali Radix)and drug by Box-Behnken response surface methodology.Results The optimal extraction conditions of Huangqi(Astragali Radix)were as follows:solid-liquid ratio 1∶6,extraction time 1 h,extraction times 3 times,the extraction rate of Huangqi(Astragali Radix)was 44.75%.The ratio of Huangqi(Astragali Radix)to medicine was based on 50 g drug mixture,29%Huangqi(Astragali Radix)concentrate/medicine mixture,0.15 g excipient 1,20 g excipient 2 and 0.2 g citric acid,and the final sensory score was 92.5 points,which was close to the predicted value,indicating that the model fit well and the optimization process was accurate and feasible.Conclusion The optimal extraction process of Huangqi(Astragali Radix)and its drug ratio optimization results were reproducible and simple,which could lay a foundation for the development of Huangqi(Astragali Radix)series products and had a certain prospect of development and application.