文献类型：期刊文献

英文题名：Potential role of N-Succinyl-Chitosan in immune reconstitution after umbilical cord blood transplantation in mice

作者：Luo, Huiying[1];Li, Juan[2];Chen, Xuan[2]

第一作者：罗慧英

通信作者：Luo, HY[1]

机构：[1]Gansu Coll Tradit Chinese Med, Dept Pharmacol, Lanzhou 730000, Peoples R China;[2]Lanzhou Univ, Hosp 1, Ctr Mol Biol, Lanzhou 730000, Peoples R China

第一机构：甘肃中医药大学

通信机构：[1]corresponding author), Gansu Coll Tradit Chinese Med, Dept Pharmacol, 35 Dingxi E Rd, Lanzhou 730000, Peoples R China.|[10735]甘肃中医药大学;

年份：2011

卷号：65

期号：8

起止页码：578

外文期刊名：BIOMEDICINE & PHARMACOTHERAPY

收录：;Scopus(收录号：2-s2.0-82755192137);WOS:【SCI-EXPANDED(收录号:WOS:000300154800008)】；

语种：英文

外文关键词：N-succinyl-chitosan; Immune reconstitution; Umbilical cord blood transplantation; CD34+cells; Proliferation

摘要：How to shorten the immune convalescence required after transplantation in recipients is still an austere medicinal problem. Speed-up recoverymeans less infection opportunity and high survival. In this study, an immune-deficient mouse model was developed by lethally irradiation to evaluate the immune reconstitution effect of N-Succinyl-Chitosan (NSC), a water soluble low molecular weight chitosan derivative, after umbilical cord blood stem cells transplantation, including hematopoiesis parameters detection, T-cell phenotype analysis and pathological comparison; effects of NSC on proliferation of umbilical cord blood stem cell has also been evaluated in vitro. By comparison and analysis, we found the combination of NSC and hematopoietic growth factors could dramatically shorten the time required for the recovery of hematopoiesis, T-cell phenotype and injured spleen after transplantation, suggesting that NSC may hasten the immune recovery and therefore may shorten the time of exposure to life-threatening opportunistic infections in transplant recipients. Moreover, we demonstrated that NSC was effective on the proliferation and clone of umbilical cord stem cell in vitro, suggesting NSC could speed up the immune reconstitution in umbilical cord blood transplantation by enlarge the number of umbilical cord blood stem cell. (C) 2010 Elsevier Masson SAS. All rights reserved.