文献类型：期刊文献

中文题名：壳聚糖金属配合物的抑菌特性及机理研究（英文）

英文题名：Antibacterial Characteristics and Mechanism of Chitosan-metal Complexes

作者：李小芳[1];冯小强[1];杨声[2];朱元成[1]

第一作者：李小芳

机构：[1]天水师范学院化学工程与技术学院,天水741001;[2]定西师范高等专科学校生化系,定西743000

第一机构：天水师范学院化学工程与技术学院,天水741001

年份：2016

卷号：28

期号：1

起止页码：17

中文期刊名：天然产物研究与开发

外文期刊名：Natural Product Research and Development

收录：CSTPCD;;北大核心:【北大核心2014】；CSCD:【CSCD2015_2016】；

基金："Qinglan" Talent Engineering Founds of Tianshui Normal University;Natural Science Foundation of China(51063006)

语种：中文

中文关键词：壳聚糖金属配合物;抑菌活性;机理

外文关键词：Chitosan-metal complexes; antibacterial activity; mechanism

摘要：制备了壳聚糖(CS)与金属离子Zn(II)、Ni(II)和Co(II)的配合物,通过红外光谱和紫外-可见吸收光谱进行了结构性能的表征。体外抑菌法研究了壳聚糖金属配合物对细菌S.aureu和E.coli的抑菌活性。结果表明:壳聚糖金属配合物的抑菌活性较壳聚糖增强,且与所含的金属离子种类有关,其中CS-Zn体现出更强的抑菌活性。因此,选CS-Zn为代表通过测定细胞内溶物的OD260nm判断细胞膜的完整性、荧光探针1-N-苯萘胺(NPN)的荧光变化来判断细胞外膜的渗透性,以研究其对大肠杆菌(E.coli.)的抑菌机理。透射电镜(TEM)结果表明CS-Zn能够破坏细菌细胞膜,使细胞内溶物溢出。
Complexes of chitosan（ CS） with Zn（ II）,Ni（ II） and Co（ II） were prepared,characterized by FT-IR and UV-vis spectra. The antibacterial activities of these complexes against Staphylococcus aureus and Escherichia coli were investigated in vitro. The results showed the antibacterial activities were dependent on the property of metal ions,and CSZn exhibited the best antibacterial activity. Hence,CS-Zn was selected to evaluate the antibacterial characteristic against E. coli. The integrity of the cell membrane and the permeability of the outer membrane（ OM） were studied respectively,by determining the release from cells of material that absorb at 260 nm and changes in the fluorescence of cells treated with the fluorescent probe 1-N-phenylnaphthylamine（ NPN）. Transmission electron microscopy（ TEM） analysis results indicated that CS-Zn disrupted bacterial cell membranes with the release of cellular content.