文献类型：期刊文献

中文题名：活血及活血解毒配伍对急性心肌梗死大鼠心肌损伤的保护作用

英文题名：Role of promoting blood circulation and its combination with detoxication in protecting AMI rats against myocardial injury

作者：马晓娟[1];郭春雨[1];张莹[2];王琳[3];殷慧军[1];史大卓[1]

第一作者：马晓娟

机构：[1]中国中医科学院西苑医院心血管中心;[2]北京中医药大学;[3]甘肃中医学院

第一机构：中国中医科学院西苑医院心血管中心

年份：2015

卷号：17

期号：9

起止页码：960

中文期刊名：中华老年心脑血管病杂志

外文期刊名：Chinese Journal of Geriatric Heart Brain and Vessel Diseases

收录：CSTPCD

基金：国家自然科学基金(81030063;81202837)

语种：中文

中文关键词：心肌梗死;活血祛瘀;肌钙蛋白I;内皮缩血管肽1;美托洛尔;中药配伍

外文关键词：myocardial infarction ; BLOOD ACT STASIS REMOV ; troponin I ; endothelin-1 ; meto-pro lol ; COMPATIBILITY （TCD）

摘要：目的观察活血及活血解毒配伍对急性心肌梗死大鼠心肌损伤的保护作用。方法选择雄性SD大鼠100只,随机分为:假手术组(等量生理盐水)、模型组(等量生理盐水)、活血组[芎芍胶囊0.39g/(kg·d)]、活血解毒组[芎芍胶囊0.39g/(kg·d)+黄连胶囊0.135g/(kg·d)]、美托洛尔组[美托洛尔2.25mg/(kg·d)],每组20只。造模24h处死大鼠,观察心肌组织病理改变,TTC法测量心肌梗死面积,ELISA法检测血清肌酸激酶同工酶(CK-MB)、肌钙蛋白I(cTnI)、内皮素1、血管性假血友病因子(vWF)、血栓调节蛋白(TM)等指标。结果与假手术组比较,模型组心肌梗死面积、CK-MB、cTnI、内皮素1、vWF、TM明显升高(P<0.01)。与美托洛尔组比较,活血解毒组cTnI明显降低[(11.88±1.98)pg/ml vs(14.13±2.02)pg/ml],内皮素1、vWF明显降低(P<0.05,P<0.01)。与活血组比较,活血解毒组cTnI、内皮素1、vWF、TM明显改善(P<0.05,P<0.01)。结论活血解毒配伍对心肌损伤发挥了较好的保护作用,显著优于单纯的活血化瘀。
Objective To study the role of promoting blood circulation and its combination with de- toxication in protecting AMI rats against myocardial injury. Methods One hundred male SD rats were randomly divided into sham operation group, model group, promoting blood circulation group,promoting blood circulation and detoxication group, and metoprolol treatment group （20 in each group）. The animals were sacrificed 24 h after a model of AMI was established. Their myo- cardial tissue lesions were observed by optical microscopy, their myocardial infarction size was measured by TIC, their serum levels of CK-MB, cTnI, ET-1, vWF, and TM were measured by ELISA. Results The myocardial infarction size was significantly larger and the serum levels of CK-MB,cTnI,ET-I,vWF,and TM were significantly higher in model group than in sham opera- tion group （P^0.01）. The serum levels of cTnI,ET-1 and vWF were significantly lower in pro- moting blood circulation and detoxication group than in metoprolol treatment group （P〈0.05, P〈0.01）. The serum levels of cTnI, ET-1, vWF, and TM were improved more signficantly in promoting blood circulation and detoxication group than in promoting blood circulation group （P〈0.05, P〈 0. 01）. Conclusion Combined promoting blood circulation and detoxication can protect AMI rats against myocardial injury,and its effect is significantly better than that of simple promoting blood circulation to remove blood stasis.