文献类型：期刊文献

英文题名：Carbon Ions Suppress Angiogenesis and Lung Metastases in Melanoma by Targeting CXCL10

作者：Li, Chengcheng[1];Zhang, Qiuning[2,3];Luo, Hongtao[2];Liu, Ruifeng[2];Feng, Shuangwu[1];Geng, Yichao[1];Wang, Lina[1];Yang, Zhen[4];Zhang, Yanying[5];Wang, Xiaohu[1,2,3,4]

第一作者：Li, Chengcheng

通信作者：Wang, XH[1]

机构：[1]Lanzhou Univ, Sch Clin Med 1, Lanzhou, Peoples R China;[2]Chinese Acad Sci, Inst Modern Phys, Lanzhou, Peoples R China;[3]Lanzhou Heavy Ions Hosp, Dept Oncol, Lanzhou, Peoples R China;[4]Gansu Univ Tradit Chinese Med, Sch Publ Hlth, Lanzhou, Peoples R China;[5]Gansu Univ Chinese Med, Lab Anim Ctr, Lanzhou, Peoples R China

第一机构：Lanzhou Univ, Sch Clin Med 1, Lanzhou, Peoples R China

通信机构：[1]corresponding author), Lanzhou Univ, Sch Clin Med 1, 1 Donggang West Rd, Lanzhou 730000, Gansu, Peoples R China.

年份：2023

卷号：200

期号：3

起止页码：307

外文期刊名：RADIATION RESEARCH

收录：;Scopus(收录号：2-s2.0-85172426059);WOS:【SCI-EXPANDED(收录号:WOS:001085733400009)】；

基金：We would like to express our gratitude to Kang Wanrong for his invaluable contribution to establishing the animal model for this research. Additionally, we extend a special thank you to Liu Zhiqiang, the physiotherapist, and Sun Shilong, the radiographer, for their assistance in irradiating the samples. We thank Home for Researchers editorial team (www.home-for-researchers.com) for language editing service. This research was made possible by the generous support of various funding sources, including the Lanzhou Innovation and Entrepreneurship Talent Project (2017-RC-23; 2020-RC-113; 2021-RC-23), National Key Research and Development Program of China (2022YFC2401500), Science and Technology Plan Project of Chengguan District of Lanzhou (2020-2-2-5), China Foundation for International Medical Exchange (Z-2017-24-2108), and Gansu Province Project of Science and Technologies (20JR10RA680).

语种：英文

摘要：Carbon-ion radiotherapy (CIRT) enhanced local control in patients with malignant melanoma. In several in vitro studies, carbon ions (C ions) have been also shown to decrease the metastatic potential of melanoma cells. CXC motif 10 (CXCL10) has been shown to play a crucial role in regulating tumor metastasis and it significantly increase in human embryonic kidney cells after heavy ion irradiations. This study sought to explore the regulatory effect of C ions on melanoma metastasis, emphasizing the role of CXCL10 in this process. To explore the potential regulatory effect of C ions on tumor metastasis in vivo, we developed a lung metastasis mouse model by injecting B16F10 cells into the footpad and subjected all mice to treatment with X rays and C ions. Subsequently, a series of assays, including histopathological analysis, enzyme-linked immunosorbent assay, real-time PCR, and western blotting, were conducted to assess the regulatory effects of C ions on melanoma. Our results showed that mice treated with C ions exhibited significantly less tumor vascularity, enhanced tumor necrosis, alleviated lung metastasis, and experienced longer survival than X-ray irradiated mice. Moreover, VEGF expression in B16F10 cells was significantly reduced by C-ion treatment, which could be alleviated by CXCL10 knockdown in vitro. Further investigations revealed that co-culturing with HUVECs resulted in a significant inhibition of proliferation, migration, and tube formation ability in the C-ion treated group, while the opposite effect was observed in the C-ion treated with si-CXCL10 group. In conclusion, our findings demonstrate that treatment with carbon-ion radiation can suppress angiogenesis and lung metastases in melanoma by specifically targeting CXCL10. These results suggest the potential utility of carbon ions in treating melanoma. (c) 2023 by Radiation Research Society