文献类型：期刊文献

中文题名：羧甲基壳聚糖-Cr(Ⅲ)配合物与鲱鱼精DNA的作用

英文题名：Interaction of Carboxymethylchitosan-Cr(Ⅲ) Complex with Herring Sperm DNA

作者：冯小强[1];李小芳[1];杨声[2]

第一作者：冯小强

机构：[1]天水师范学院生命科学与化学学院,天水741001;[2]定西师范高等专科学校,定西743000

第一机构：天水师范学院生命科学与化学学院,天水741001

年份：2014

卷号：26

期号：11

起止页码：1757

中文期刊名：天然产物研究与开发

外文期刊名：Natural Product Research and Development

收录：CSTPCD;;北大核心:【北大核心2011】；CSCD:【CSCD2013_2014】；

基金：天水师范学院"青蓝"人才工程和中青年科研项目(TSA1305;TSA1307)

语种：中文

中文关键词：羧甲基壳聚糖-Cr(III);鲱鱼精DNA;循环伏安法;紫外光谱;相互作用

外文关键词：CMC-Cr; herring sperm DNA; cyclic voltammetry; UV-Vis spectrum; interaction

摘要：采用循环伏安法和紫外光谱法研究了羧甲基壳聚糖-Cr(III)配合物与鲱鱼精DNA之间的作用方式。结果发现:(1)羧甲基壳聚糖-Cr(III)的存在导致Fe(CN)3-6/4-探针分子氧化还原峰电流下降,式量电位正移,显示羧甲基壳聚糖-Cr(III)和Fe(CN)3-6/4-与DNA之间存在竞争性作用,说明羧甲基壳聚糖-Cr(III)通过插入方式与DNA相互作用的;(2)中性红的加入导致羧甲基壳聚糖-Cr(III)/DNA体系的特征吸收峰发生增色效应、峰位红移并伴有等吸收点出现,这进一步表明羧甲基壳聚糖-Cr(III)是以插入方式与鲱鱼精DNA发生了相互作用,导致DNA分子的构象变化,并且CMC-Cr与DNA的结合比n(CMC-Cr):n(DNA)为3∶1。
The interaction between carboxymethylehitosan-Cr （Ⅲ） Complex （CMC-Cr） and herring sperm DNA were investigated by cyclic voltammetry and UV-Vis spectra. The results indicated：Firstly,the peak current of probe molecule Fe （CN）3-/6 at Au/DNA electrodes obviously decreased and the peak potential shifted positively because of the exist- ences of CMC-Cr,which showed that there was a competitive effect between CMC-Cr and Fe （CN）3-/6 /4 with herring sperm DNA,and revealed that a binding mode intercalation was interacted between CMC-Cr and herring sperm DNA. Secondly, the absorption intensity of CMC-Cr/DNA system increased with the adding of neutral red （NR）, the maximum absorption peak had a red shifted and appeared equal absorption point, which further illustrated that a binding mode intercalation was interacted between CMC-Cr and herring sperm DNA, thus led to the change of DNA conformation. The combining ratio of n（CMC-Cr） and n（DNA） was 3： 1.