文献类型：期刊文献

中文题名：β-蜕皮甾酮调控BMSCs自噬促进成骨分化的作用

英文题名：Effects ofβ-ecdysterone in regulating BMSCs autophagy and promoting osteogenic differentiation

作者：汪可欣[1];白敏[2];宋冰[1,3];郭超[1,3];张延英[2,3];邢尚曼[1];宋文静[1];曹婷婷[1];汪永锋[1,3,4]

第一作者：汪可欣

机构：[1]甘肃中医药大学第一临床医学院,甘肃兰州730000;[2]甘肃中医药大学基础医学院,甘肃兰州730000;[3]甘肃省实验动物行业技术中心,甘肃兰州730000;[4]甘肃医学院基础医学院,甘肃平凉744000

第一机构：甘肃中医药大学临床医学院

年份：2025

卷号：41

期号：4

起止页码：527

中文期刊名：中国临床药理学杂志

外文期刊名：The Chinese Journal of Clinical Pharmacology

收录：;北大核心:【北大核心2023】；

基金：甘肃省科技重点研发计划基金资助项目(23YFFA0068);甘肃省教育厅高等学校科研基金资助项目(2023A-080);甘肃中医药大学成果转化培育基金资助项目(2023CGZH-21);甘肃中医药大学创新创业基金项目;甘肃省中医药管理局基金资助项目(GZKP-2023-19)。

语种：中文

中文关键词：β-蜕皮甾酮;5′-腺嘌呤核苷酸依赖的蛋白激酶/雷帕霉素靶蛋白通路;骨髓间充质干细胞;成骨分化;自噬

外文关键词：β-ecdyssterone;adenosine 5'-monophosphate kinase-activated protein/mammalian target of rapamycin pathway;bone marrow mesenchymal stem cells;osteogenic differentiation;autophagy

摘要：目的基于5′-腺嘌呤核苷酸依赖的蛋白激酶(AMPK)/哺乳动物雷帕霉素靶蛋白(mTOR)通路调控自噬探讨β-蜕皮甾酮对大鼠骨髓间充质干细胞(BMSCs)成骨分化的干预作用及机制。方法将BMSCs细胞分为对照组(正常培养)和低、中、高剂量组(用0.01、0.10和1.00μmol·L^(-1)的β-蜕皮甾酮进行干预)。用实时荧光定量聚合酶链反应(qRT-PCR)法测定细胞基因的表达,用免疫荧光(IF)法测定细胞蛋白相对表达水平。结果对照组和低、中、高剂量组的碱性磷酸酶(ALP)mRNA相对表达水平分别为1.01±0.14、1.22±0.05、1.28±0.05和1.59±0.20,Runt相关转录因子2(RUNX2)mRNA相对表达水平分别为1.00±0.07、1.45±0.07、2.11±0.32和4.67±1.45,磷酸化AMPK蛋白相对表达水平分别为0.07±0.01、0.11±0.01、0.06±0.01和0.18±0.01,选择性自噬接头蛋白(p62/SQSTM1)相对表达水平分别为1.72±0.02、1.67±0.02、0.94±0.01和0.04±0.01,p-mTOR蛋白相对表达水平分别为0.66±0.01、0.40±0.01、0.42±0.01和0.04±0.01,微管相关蛋白轻链3(LC3B)蛋白相对表达水平分别为0.07±0.01、0.20±0.01、0.87±0.05和1.27±0.04。低、中、高剂量组的上述指标与对照组比较,在统计学上差异均有统计学意义(P<0.05,P<0.001)。结论β-蜕皮甾酮能够通过激活AMPK/mTOR通路调控BMSCs自噬促进其成骨分化。
ObjectiveTo investigate the intervention effect and mechanism ofβ-ecdyssterone on the osteogenic differentiation of rat bone marrow mesenchymal stem cells(BMSCs)based on the regulation of autophagy by the adenosine 5'-monophosphate kinase-activated protein(AMPK)/mammalian target of rapamycin(m TOR).Methods BMSCs cells were divided into control group(normal culture),low-,middle-and high-dose groups(intervened with 0.01,0.10 and1.00μmol·L^(-1)ecdysterone).The expression of cell genes was determined by real-time quantitative polymerase chain reaction(qRT-PCR);and the relative expression level of cell proteins was determined by immunofluorescence(IF).Results The relative expression levels of alkaline phosphatase(ALP)mRNA in the control group,low-,middle and high-dose groups were 1.01±0.14,1.22±0.05,1.28±0.05,1.59±0.20;the relative protein expression levels of Runt-related transcription factor 2(RUNX2)mRNA were1.00±0.07,1.45±0.07,2.11±0.32,4.67±1.45;the relative protein expression levels of phosphorylated AMPK protein were 0.07±0.01,0.11±0.01,0.06±0.01,0.18±0.01;and the relative protein expression levels of Sequestosome 1(p62/SQSTM1)protein were 1.72±0.02、1.67±0.02、0.94±0.01、0.04±0.01;the relative protein expression levels of p-m TOR protein were 0.66±0.01,0.40±0.01,0.42±0.01,0.04±0.01;and the relative protein expression levels of microtubule-associated protein 1 light chain 3 beta(LC3B)protein were0.07±0.01,0.20±0.01,0.87±0.05,1.27±0.04,respectively.There were statistically significant differences between the low-,middle-,and high-dose groups and the control group(P<0.05,P<0.001).Conclusionβ-ecdyssterone can regulate autophagy and promote osteogenic differentiation of BMSCs by activating the AMPK/m TOR pathway.