文献类型：期刊文献

中文题名：热补针法对寒证类风湿关节炎模型家兔滑膜组织代谢物的影响

英文题名：Effects of Heat-reinforcing Needling on Synovial Tissues of Rheumatoid Arthritis Rabbits with Cold Syndrome

作者：杜小正[1];袁博[1];王金海[2];方晓丽[1];张星华[1];田亮[1];张婷卓[1];李兴兰[1];曾华宗[3]

第一作者：杜小正

机构：[1]甘肃中医药大学,甘肃兰州730000;[2]兰州大学第二医院,甘肃兰州730030;[3]上海聚类生物科技有限公司,上海200433

第一机构：甘肃中医药大学

年份：2017

卷号：24

期号：2

起止页码：55

中文期刊名：中国中医药信息杂志

外文期刊名：Chinese Journal of Information on Traditional Chinese Medicine

收录：CSTPCD;;CSCD:【CSCD_E2017_2018】；

基金：国家自然科学基金(81260558);国家中医药管理局甘肃郑氏针法学术流派传承工作室项目(2305135901);甘肃省自然科学基金(1208RJZA185);兰州市科技计划项目(2014-10247)

语种：中文

中文关键词：热补针法;类风湿关节炎;滑膜组织;代谢组学;气相色谱-质谱法;兔

外文关键词：heat-reinforcing needling; rheumatoid arthritis; synovial tissue; metabolomics; GC-MS; rabbits

摘要：目的通过测定寒证类风湿关节炎(RA)模型家兔滑膜组织内源性代谢物,探讨热补针法治疗RA的特异性机制。方法采用卵蛋白诱导联合低温冷冻法复制寒证RA模型。将40只清洁级青紫蓝家兔随机分为正常组、模型组、平补平泻组、捻转补法组、热补针法组,每组8只。除正常组和模型组外,其余各组分别施以平补平泻、捻转补法、热补针法针刺"足三里",1次/d,留针30 min,连续7 d。干预结束后分离家兔膝关节滑膜组织,应用气相色谱-四级杆飞行时间质谱采集各组滑膜代谢物谱图,利用主成分分析和偏最小二乘判别分析对数据进行统计。结果与正常组比较,模型组家兔滑膜组织代谢物变化主要体现于三羧酸循环、糖类及脂肪酸代谢相关物质增高(P<0.05);与模型组比较,各针刺组家兔滑膜组织代谢物变化主要体现于上述各种代谢相关物质降低(P<0.05),且热补针法组三羧酸循环与糖代谢相关物质明显低于平补平泻组和捻转补法组(P<0.05)。结论热补针法治疗RA的特异性体现在对三羧酸循环及糖代谢通路的调控。
Objective To measure the endogenous metabolites in synovial tissues of rheumatoid arthritis（RA） rabbits with cold syndrome; To investigate the specificity mechanisms of heat-reinforcing needling for RA. Methods A total of forty healthy purple blue rabbits were randomly allocated to normal group, model group, reinforcing-reducing needling group（RRN）, twirling-reinforcing needling group（TRN） and heat-reinforcing needling group（HRN） 8 rabbits in each group. Arthritis rabbits cold model was made with ovalbumin and freezing. Except for normal groupand model group, RRN was given acupuncture of reinforcing-reducing needling at Zusanli（ST36）, TRN was given acupuncture of twirling-reinforcing needling at Zusanli（ST36）, HRN was administrated acupuncture of heat-reinforcing needling at Zusanli（ST36）, once a day and retaining 30 min, a total of seven days. Fresh synovial tissues of rabbits knee joints were extracted after the intervention, then GC-Q/TOF-MS technology were employed to evaluate metabolic profiles. Results The synovial tissues metabolites of TCA cycle, carbohydrate and fatty acid metabolism in model group mainly increased compared with normal group（P〈0.05）; The synovial tissues metabolites of various kinds above-mentioned decreased compared with model group in all intervention of acupuncture group（P〈0.05）, and the metabolites of TCA cycle and carbohydrate metabolism in HRN were obviously less than that of RRN and TRN（P〈0.05）. Conclusion The specificity of heat-reinforcing needling for RA manifests in regulation of TCA cycle and carbohydrate metabolism.