文献类型：期刊文献

中文题名：雌激素与植物雌激素对去势鼠子宫内膜渗出的影响及机制探讨(英文)

英文题名：Effects of estrogen and phytoestrogens on endometrial leakage in ovariectomized rats and the related mechanisms

作者：李红芳[1,2];段颖[1,3];汪龙德[4];田治峰[1];邱小青[1];张英福[1];张华[1,2];杨丽娜[1,2]

第一作者：李红芳

机构：[1]兰州大学基础医学院生理学教研室;[2]甘肃省新药临床前研究重点实验室,兰州730000;[3]甘肃省肿瘤医院,兰州730050;[4]甘肃中医学院附属医院,兰州730020

第一机构：兰州大学基础医学院生理学教研室

年份：2013

期号：1

起止页码：8

中文期刊名：生理学报

外文期刊名：Acta Physiologica Sinica

收录：MEDLINE(收录号：23426508);CSTPCD;;Scopus(收录号：2-s2.0-84892418098);北大核心:【北大核心2011】；CSCD:【CSCD2013_2014】；PubMed;

基金：supported by Research Fund from Lanzhou Science and Technology Bureau,China(No.2010-1-88);the Natural Science Foundation of Gansu Province,China(No.1010RJZA111);Research Fund from Administration of Traditional Chinese Medicine and Chinese Herb,Gansu Province,China(No.GZK-2010-38)

语种：中文

中文关键词：雌激素;植物雌激素;血管通透性;白细胞渗出;血管内皮生长因子;细胞因子

外文关键词：estrogen; phytoestrogen; capillary permeability; leukocyte leakage; vascular endothelial growth factor; cytokines

摘要：植物雌激素属于植物衍生的非甾体化合物,可与雌激素受体结合模拟雌激素的作用,其对人类健康的潜在有益作用已成为目前国内外研究的热点。然而,植物雌激素对生殖系统的副作用研究报道较少,本文利用免疫组化和蛋白免疫印迹技术,比较17β-雌二醇(17β-estradiol,E2)、黄体酮(progesterone,P4)以及植物雌激素染料木素(genistein,Gen)、白藜芦醇(resveratrol,Res)、根皮素(phloretin,Phl)对去卵巢大鼠子宫内膜血管渗出和血管通透性的影响。以假手术大鼠为正常对照,每天分别给去势鼠皮下注射E2、E2合并P4、P4、Gen、Res或Phl,连续21d。大鼠子宫组织HE染色显示,去势鼠皮下给予E2、E2和P4联合处理均可诱发嗜酸性粒细胞的渗出;免疫组化和Western blot分析显示,这些去势鼠同时伴有子宫内膜VEGF、NF-κB和TNF-α表达明显上调;而Gen、Res、Phl三种植物雌激素处理后未发现子宫内膜嗜酸性粒细胞渗出及VEGF、NF-κB和TNF-α表达明显改变。结果表明:E2单独使用或E2和P4联用可通过上调VEGF、TNF-α和NF-κB的表达提高子宫内膜血管通透性,诱发去势鼠子宫内膜嗜酸性粒细胞的渗出,而同剂量的植物雌激素Gen、Res和Phl则无明显作用。
Phytoestrogens, a group of plant-derived non-steroidal compounds that can behave as estrogens by binding to estrogen re- ceptors, have drawn great attention for their potentially beneficial effects on human health. However, there are few studies investigat- ing the potential side effects of phytoestrogens on the reproductive system. The present study was to elucidate the effects of 17β-estradiol （E2）, progesterone （P4）, and phytoestrogens genistein （Gen）, resveratrol （Res）, and phloretin （Phl） on eosinophilic infil- tration of the ovariectomized rat uterus and endometrial vascular permeability, and to analyze the underlying mechanisms. The ova- riectomized rats received daily subcutaneous injections of E2, E2＋P4, P4, Gen, Res, Phl, or an equivalent volume of vehicle for 21 days, and sham-operated animals （Sham rats） were used as the controls. Hematoxylin-eosin staining revealed a marked increase in uterine eosinophilic infiltrations in ovariectomized rats treated with E2, E2＋P4 or P4, which was associated with increased expression of vascular endothelial growth factor （VEGF）, nuclear factor-κB （NF-κB）, and tumor necrosis factor-α （TNF-α） proteins as deter- mined by immunohistochemical and Western blot analysis. However, all three phytoestrogens had no markedly effect on the uterine eosinophilic infiltration and the expressions of VEGF, NF-κB, and TNF-α in the uterus of ovariectomized rats. Our data demonstrate that E2 alone or in combination with P4 increases uterine eosinophilic infiltration which is related with vascular hyperpermeability caused by VEGF, NF-κB and TNF-α, whereas phytoestrogens Gen, Res, and Phl, have no such an effect.