文献类型：期刊文献

英文题名：Human serum albumin-derived biosensor for visualizing copper(II) and hydrogen sulfide interactions in cancer cells

作者：An, Yong[1];Zhou, Yucen[2];Zhang, Linghua[3];Qiang, Zhengze[3];Li, Lepeng[1];Wu, Hao[1];Li, Yaling[2];Wang, Peng[4]

第一作者：An, Yong

通信作者：Wu, H[1];Li, YL[2];Wang, P[3]

机构：[1]Gansu Univ Chinese Med, Sch Clin Med 1, Lanzhou 730000, Gansu, Peoples R China;[2]Gansu Univ Chinese Med, Gansu Univ Key Lab Mol Med & Chinese Med Prevent &, Lanzhou 730000, Gansu, Peoples R China;[3]Gansu Univ Chinese Med, Coll Pharm, Lanzhou 730000, Gansu, Peoples R China;[4]China West Normal Univ, Chem Synth & Pollut Control Key Lab Sichuan Prov, Coll Chem & Chem Engn, Nanchong 637009, Peoples R China

第一机构：甘肃中医药大学

通信机构：[1]corresponding author), Gansu Univ Chinese Med, Sch Clin Med 1, Lanzhou 730000, Gansu, Peoples R China;[2]corresponding author), Gansu Univ Chinese Med, Gansu Univ Key Lab Mol Med & Chinese Med Prevent &, Lanzhou 730000, Gansu, Peoples R China;[3]corresponding author), China West Normal Univ, Chem Synth & Pollut Control Key Lab Sichuan Prov, Coll Chem & Chem Engn, Nanchong 637009, Peoples R China.|[10735]甘肃中医药大学;

年份：2026

卷号：82

起止页码：999

外文期刊名：JOURNAL OF ADVANCED RESEARCH

收录：;Scopus(收录号：2-s2.0-105012360130);WOS:【SCI-EXPANDED(收录号:WOS:001717881100001)】；

基金：This work were supported by the National Natural Science Foundation of China (NO. 82303568 and N0. 22206154) , Gansu Provincial Natural Science Foundation (NO. 23JRRA1216) , Gansu Provincial University Innovation Foundation (NO. 2023QB-085) , the Introduced Talent Research Start-up Fund (NO. 2023-YJRC-16) , Scientific Research and Innovation Fund (NO. 2021-KCYB-07) of Gansu University of Chinese Medicine.

语种：英文

外文关键词：Human serum albumin; Hydrogen sulfide; Mechanism visualization; Redox; Cu2+ ions

摘要：Introduction: Accurate monitoring of Cu2+ and H2S levels is one of the most reliable methods for diagnosing tumors and assessing treatment efficacy. However, the development of fluorescent probes capable of detecting Cu2+ and H2S in cancer cells against interference from the tumour microenvironment remains a challenge. Objective: This study aimed to develop a novel peptide-based biosensor for monitoring Cu2+ and H2S in cancer cells and visualizing the effects of Cu2+-H2S interactions on redox homeostasis. Methods: First, we designed and synthesized the sensor NBD-DAH (NBD-Asp-Ala-His) by mimicking the Cu2+ binding site of human serum albumin (HSA). Second, we evaluated the specificity, sensitivity, and resistance of NBD-DAH to interference from the biological environment in the sequential detection of Cu2+ and H2S. Third, we investigated the binding sites and sensing mechanism between NBD-DAH and Cu2+. Finally, we evaluated the potential and biosafety of NBD-DAH for monitoring Cu2+ and H2S, visualizing their interactions in cancer cells, and exploring the effects and potential mechanisms of these interactions on redox homeostasis. Results: NBD-DAH can specifi on" fluorescence response. Be rapid response, the sensor resists interferences from the complex tumor microenvironment and enables reliable detection of Cu2' and H2S level changes in cancer cells. Furthermore, live-cell imaging with NBD-DAH revealed a molecular mechanism in which H2S specifically binds to Cu2' in cancer cells, leading to the depletion of superoxide di Conclusion: We developed a novel biosensor, NBD-DAH, and successfully used it to monitor Cu2' and H2S levels and visualize their interactions in cancer cells. The interaction between Cu2' and H2S influenced intracellular redox homeostasis, potentially through its effect on SOD1 activity. cally and sensitively detect Cu2' and H2S in sequence through an "on-off-cause of its high cell membrane permeability, broad pH compatibility, and smutase 1 (SOD1) activity and subsequent regulation of redox homeostasis. (c) 2025 The Author(s). Pub lished by Elsevier B.V. on behalf of Cairo University. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).