文献类型：期刊文献

中文题名：免疫致敏结合局部乙酸刺激法建立大鼠溃疡性结肠炎模型

英文题名：Establishment of a Rat Model of Ulcerative Colitis by Immunological Sensitization Combined with Local Acetic Acid Irritation

作者：吴玉泓[1,2];李海龙[2];段永强[2];吴建军[2];王宏伟[3];魏彦明[1]

第一作者：吴玉泓

机构：[1]甘肃农业大学动物医学院,兰州730070;[2]甘肃中医学院基础部,兰州730000;[3]甘肃中医学院附属医院检验科,兰州730020

第一机构：甘肃农业大学动物医学院,兰州730070

年份：2010

卷号：18

期号：1

起止页码：65

中文期刊名：中国实验动物学报

外文期刊名：Acta Laboratorium Animalis Scientia Sinica

收录：CSTPCD;;CSCD:【CSCD2011_2012】；

基金：甘肃省中医药管理局资助项目(编号:06-GZK-22)

语种：中文

中文关键词：结肠黏膜组织致敏法;乙酸局部刺激法;溃疡性结肠炎;复合动物模型

外文关键词：Immunological sensitization; Local irritation; acetic acid; Ulcerative colitis; Model; establishment; Rat

摘要：目的结肠黏膜组织致敏法加乙酸局部刺激法建立复合大鼠溃疡性结肠炎模型。方法30只Wistar大鼠随机分为正常对照组、模型对照组、用药组(各10只),用结肠黏膜组织致敏法加乙酸局部刺激法建立大鼠复合溃疡性结肠炎模型后,用药组给予SASP灌胃,模型对照组和正常对照组给予生理盐水,均灌胃2周后处死大鼠,分离其结肠组织和血清。生化法检测各组结肠中MDA、SOD、GSH-PX、NO、TNOS和iNOS值,放免法测定大鼠血清及结肠IL-4及TNF-α含量变化,酶联免疫法测定大鼠血清IFN-γ含量变化。结果与空白对照组比较,模型组大鼠结肠组织MDA、NO、TNOS及iNOS含量升高,SOD、GSH-Px水平显著降低。与模型组比较,SASP组SOD、GSH-Px计数水平显著升高,组织MDA、NO、TNOS及iNOS含量降低。模型组血清和结肠IL-4含量较对照组明显降低,而模型大鼠血清和结肠TNF-α含量明显升高,模型组大鼠血清IFN-γ含量明显升高。SASP组IL-4含量升高,TNF-α和IFN-γ含量明显降低。结论改进的复合造模方法,可以较好的模拟人类UC病变的慢性活动性特点,适合药效观察和评价。
Objective To establish a rat model of ulcerative colitis by immunological sensitization combined with local acetic acid irritation.Methods Thirty healthy 2-3-month-old Wistar rats were randomly divided into 3 groups：normal control group,model group and SASP-treated model group（n = 10 rats per group）.The ulcerative colitis in the model group and positive group was developed by immunological sensitization with rabbit colon mucosal proteins and local irritation with 5% acetic acid.The rats of the model and positive groups were fed with sulfasalazine,and rats of the control group fed with normal saline.The rats of positive control group were treated with sulfasalazine.Two weeks later,all rats were killed and colon mucosal and blood samples were collected.The concentrations of NO,NOS,MDA,SOD and GSH-Px in the colonic mucosal homogenate were measured by biochemistry,the contents of IL-4 and TNF-α were measured by RIA,and the serum concentration of IFN-γ was measured by ELISA.Results Compared with the normal control group,the concentration of MDA,NO,NOS in the colonic mucosa was increased,and SOD and GSH-Px levels significantly decreased in the model group.Compared with the model group,the concentrations of MDA,NO,and NOS in the colonic mucosal tissue were decreased,and the levels of SOD and GSH-Px decreased in the SASP-treated model group.The contents of IL-4 in the serum and colonic mucosal homogenate of model group were significantly lower,and the contents of TNF-α in the serum and colonic mucosal homogenate were significantly increased than those of the normal control group.The IL-4 levels in the serum and colonic mucosal homogenate was increased,and that of TNF and IFN-γ significantly decreased in the SASP-treated model group,compared with those of the normal control group.Conclusion The rat model of ulcerative colitis established by immunological sensitization combined with local acetic acid irritation,as described in the present study,can better simulate the characteristic chronic alterations in human ulcerative colitis,and may better serve studies and evaluation of treatment of this disease.