文献类型：期刊文献

中文题名：心理应激致孕鼠乳腺发育不良及雌/孕激素和受体水平异常

英文题名：Mammary dysplasia and abnormal estrogen/progesterone or their receptor expression induced by psychological stress in pregnant rats

作者：王瑞琼[1];吴国泰[2];刘峰林[2];魏彦明[1];吴玉泓[2]

第一作者：王瑞琼

机构：[1]甘肃农业大学,兰州730070;[2]甘肃中医学院甘肃省中药药理与毒理学重点实验室,兰州730000

第一机构：甘肃农业大学,兰州730070

年份：2015

卷号：23

期号：3

起止页码：272

中文期刊名：中国实验动物学报

外文期刊名：Acta Laboratorium Animalis Scientia Sinica

收录：CSTPCD;;北大核心:【北大核心2014】；CSCD:【CSCD_E2015_2016】；

基金：国家自然科学基金项目(No.31272600)

语种：中文

中文关键词：心理应激;Wistar大鼠;乳腺发育;雌二醇;孕酮

外文关键词：Psychological stress;;Wistar rat;;Mammary development;;Estradiol;;Progesterone

摘要：目的研究心理应激对孕鼠乳腺发育的影响,评价相关激素及其受体的表达水平。方法妊娠期Wistar大鼠随机分为对照组和实验组,实验组大鼠15 d内随机施加噪音、束缚、昼夜颠倒、冰水游泳和夹尾致痛5种不可预见性应激刺激。观察大鼠乳房外观变化,测量乳头直径和高度并计算乳房系数,光密度法测定乳腺组织DNA和RNA水平,放射免疫法测定血浆和乳腺组织雌激素(E2)、孕酮(P)、生长激素(GH)及催乳素(PRL)水平,放射配基结合分析法测定雌二醇、孕酮受体的最大结合容量(Bmax)和解离常数(Kd),乳房组织H-E染色,光镜下观察组织形态变化。结果与对照组比较,实验组大鼠乳头直径和高度减小、乳房重量减轻、乳房系数减小(P<0.05,P<0.01),乳房组织DNA、RNA及RNA/DNA均明显减小(P<0.05,P<0.01),血浆E2、P和GH水平明显下降(P<0.05,P<0.01),组织匀浆E2和GH水平明显降低(P<0.05,P<0.01),E2受体和P受体Bmax明显降低、Kd均明显升高(P<0.05,P<0.01);乳腺小叶、小叶腺泡数目减少、腺泡直径明显降低(P<0.05)。结论心理应激能导致孕鼠乳腺发育不良,引起雌、孕激素水平及其受体表达异常。
Objective To study the effects of psychological stress on breast development and abnormal levels of related hormones or their receptors in pregnant rats. Methods Pregnant Wistar rats were randomly divided into control group and experimental group. The experimental group rats were given unpredictable stress stimuli for 15 days,including noise,restraint,reversal of day and night,swimming in cold water and tail pinch-induced pain. Gross changes of the breast were observed,the diameter and height of breast nipple was measured,and breast coefficient was calculated. DNA and RNA levels in the breast tissues were determined by optical density measurement. The estrogen( E2),progesterone( P),growth hormone( GH) and prolactin( PRL) levels in plasma and breast tissues were determined by radioimmunoassay. Bmax and Kd of estradiol and progesterone receptors were measured by radolig and bindng assay. Morphological changes of the breast tissues were examined by light microscopy using HE staining. Results Compared with the control group,the nipple diameter and height,breast weight and breast coefficient were significantly decreased in the experimental group( P < 0. 05,P < 0. 01),the levels of DNA,RNA and RNA / DNAratio in the breast tissue were significantly decreased( P < 0. 05,P < 0. 01),the levels of plasma E2,P and GH were significantly decreased( P < 0. 05,P < 0. 01),the levels of E2 and GH in the breast tissue homogenate were decreased also( P < 0. 05,P< 0. 01),the Bmaxs of E2 receptor and P receptor were decreased,but their Kds increased significantly( P < 0. 05,P < 0. 01),and the number of lobules and diameter of acini were significantly decreased( P < 0. 05). Conclusions Psychological stress can lead to breast dysplasia and abnormal levels of estrogen and progesterone and their receptors in pregnant rats.