文献类型：期刊文献

英文题名：Sodium Hydrosulfide Protects Rats from Hypobaric-Hypoxia-Induced Acute Lung Injury

作者：Wang, Renjie[1,2];Ma, Shuhe[1,2];Yang, Jun[1,2];Luo, Kai[1,2];Qian, Qingyuan[2,3];Pan, Jinchao[2,4];Liang, Keke[1,2];Wang, Yihao[2];Gao, Yue[2,5];Li, Maoxing[2,5]

第一作者：Wang, Renjie

通信作者：Gao, Y[1];Li, MX[1];Gao, Y[2];Li, MX[2]

机构：[1]Gansu Univ Chinese Med, Coll Pharm, Lanzhou 730000, Peoples R China;[2]Beijing Inst Radiat Med, Dept Pharmaceut Sci, Beijing 100850, Peoples R China;[3]Lanzhou Univ, Coll Pharm, Lanzhou 730000, Peoples R China;[4]Beijing Univ Technol, Fac Environm & Life, Beijing 100083, Peoples R China;[5]Natl Key Lab Kidney Dis, Beijing 100850, Peoples R China

第一机构：甘肃中医药大学药学院（西北中藏药协同创新中心办公室）

通信机构：[1]corresponding author), Beijing Inst Radiat Med, Dept Pharmaceut Sci, Beijing 100850, Peoples R China;[2]corresponding author), Natl Key Lab Kidney Dis, Beijing 100850, Peoples R China.

年份：2024

卷号：25

期号：19

外文期刊名：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

收录：;Scopus(收录号：2-s2.0-85206345562);WOS:【SCI-EXPANDED(收录号:WOS:001332372000001)】；

基金：This project was funded by the Traditional Chinese Medicine Innovation Team and Talent Support Program Project from the National Administration of Traditional Chinese Medicine (Funder Yue Gao, Funding number ZYYCXTDD-202207).

语种：英文

外文关键词：hydrogen sulfide; high-altitude hypoxia; acute lung injury; proteomics; antioxidant; anti-inflammatory; anti-apoptotic

摘要：Hydrogen sulfide (H2S), as a key gas signaling molecule, plays an important role in regulating various diseases, with appropriate concentrations providing antioxidative, anti-inflammatory, and anti-apoptotic effects. The specific role of H2S in acute hypoxic injury remains to be clarified. This study focuses on the H2S donor sodium hydrosulfide (NaHS) and explores its protective effects and mechanisms against acute hypoxic lung injury. First, various mouse hypoxia models were established to evaluate H2S's protection in hypoxia tolerance. Next, a rat model of acute lung injury (ALI) induced by hypoxia at 6500 m above sea level for 72 h was created to assess H2S's protective effects and mechanisms. Evaluation metrics included blood gas analysis, blood routine indicators, lung water content, and lung tissue pathology. Additionally, LC-MS/MS and bioinformatic analyses were combined in performing quantitative proteomics on lung tissues from the normoxic control group, the hypoxia model group, and the hypoxia model group with NaHS treatment to preliminarily explore the protective mechanisms of H2S. Further, enzyme-linked immunosorbent assays (ELISA) were used to measure oxidative stress markers and inflammatory factors in rat lung tissues. Lastly, Western blot analysis was performed to detect Nrf2, HO-1, P-NF-kappa B, NF-kappa B, HIF-1 alpha, Bcl-2, and Bax proteins in lung tissues. Results showed that H2S exhibited significant anti-hypoxic effects in various hypoxia models, effectively modulating blood gas and blood routine indicators in ALI rats, reducing pulmonary edema, improving lung tissue pathology, and alleviating oxidative stress, inflammatory responses, and apoptosis levels.