文献类型：期刊文献

中文题名：野生与栽培黄管秦艽7种指标成分的比较研究

英文题名：Comparative Study on 7 Indicative Constituents of Wild and Cultivated Gentiana officinalis

作者：杨燕梅[1];马晓辉[1];吕培霖[2];卢有媛[1];林丽[1,3];王振恒[1,3];晋玲[1,3];朱田田[1,3]

第一作者：杨燕梅

机构：[1]甘肃中医药大学药学院,兰州730000;[2]解放军第25医院药剂科,甘肃酒泉735000;[3]中(藏)药资源研究所,兰州730000

第一机构：甘肃中医药大学药学院（西北中藏药协同创新中心办公室）

年份：2016

卷号：27

期号：19

起止页码：2618

中文期刊名：中国药房

外文期刊名：China Pharmacy

收录：CSTPCD;;北大核心:【北大核心2014】；

基金：中央本级重大增减支项目子课题(No.20603020212;No.20603020110);2015年甘肃省高等学校科研项目(No.2015A-098)

语种：中文

中文关键词：野生;栽培;黄管秦艽;超高效液相色谱法;指标成分

外文关键词：Wild; Cultivated; Gentiana officinalis; UPLC method; Indicative constituents

摘要：目的：比较野生与栽培黄管秦艽中马钱苷酸、獐牙菜苦苷、6′-O-β-D-葡萄糖基龙胆苦苷、龙胆苦苷、獐牙菜苷、异荭草苷和异牡荆苷7种指标成分的含量,为黄管秦艽的合理使用提供依据。方法：采用超高效液相色谱法。色谱柱为ACQUITY UPLC（R）BEH C_（18）（50 mm×2.1 mm,1.7μm）,流动相为甲醇-0.04%磷酸溶液（梯度洗脱）,流速为0.3 ml/min,检测波长为242 nm,柱温为30℃。结果：马钱苷酸、獐牙菜苦苷、6′-O-β-D-葡萄糖基龙胆苦苷、龙胆苦苷、獐牙菜苷、异荭草苷和异牡荆苷进样量分别在2.1～537.1、1.05～270、0.92～236、11.1～2 830、0.75～192、0.167～102、0.216～52.80μg范围内与峰面积积分值线性关系良好（r≥0.999 5）,平均回收率为97.72%～99.84%（RSD≤3.39%,n=6）。野生黄管秦艽中马钱苷酸、獐牙菜苦苷、6′-O-β-D-葡萄糖基龙胆苦苷、龙胆苦苷、獐牙菜苷、异荭草苷的含量略高于栽培黄管秦艽,而异牡荆苷含量略低于栽培黄管秦艽,但差异均无统计学意义（P〉0.05）。野生与栽培品中龙胆苦苷与马钱苷酸含量总和均高于2.5%,达到2015年版《中国药典》（一部）要求。结论：野生与栽培黄管秦艽7种指标成分的含量差异无统计学意义,且药典规定成分的含量都合格。
OBJECTIVE：To compare the contents of loganic acid,swertiamarin,6′-O-β-D-glucosyl gentiopicroside,gentiopicroside,sweroside,isoorientin and isovitexin in wild and cultivated Gentiana officinalis,and to provide basis for rational use of G.officinalis.METHODS：UPLC method was adopted.The separation was performed on ACQUITY UPLC#174;BEH C18column（50mm×2.1 mm,1.7 μm）with mobile phase consisted of methanol-0.04% phosphoric acid（gradient elution）at the flow rate of 0.3ml/min.The detection wavelength was set at 242 nm,and column temperature was 30 ℃.RESULTS：For loganic acid,swertiamarin,6′-O-β-D-glucosyl gentiopicroside,gentiopicroside,sweroside,isoorientin and isovitexin,a good linearity was obtained in the range of 2.1-537.1 μg,1.05-270 μg,0.92-236 μg,11.1-2 830 μg,0.75-192 μg,0.167-102 μg,0.216-52.80 μg（r≥0.999 5）,respectively.Their average recoveries were 97.72%-99.84%（RSD≤3.39%,n=6）.The contents of loganic acid,swertiamarin,6′-O-β-D-glucosyl gentiopicroside,gentiopicroside,sweroside and isoorientin in the wild sample were higher than in the cultivated;the content of isovitexin was lower than the cultivated,but there was no statistical significance（P〈0.05）.The sum of gentiopicroside and loganin acid content were all higher than 2.5% in both wild and cultivated samples,and met the requirements of 2015 edition of Chinese Pharmacopoeia（first part）.CONCLUSIONS：The content difference of 7 indicative constituents in wild and cultivated G.officinalis is not statistically significant,and the indicative constituents of the pharmacopoeia is qualified.