文献类型：期刊文献

中文题名：补肾活血复方藤黄健骨胶囊通过激活Sirt1/FoxO3a对抗去势大鼠骨质疏松研究

英文题名：Bushen Huoxue Fufang Tenghuang Jiangu Capsule Attenuates Postmenopausal Osteoporosis via Activating Sirt1/FoxO3a in Ovariectomized Rats

作者：王芳[1];贾雪茹[1];张捷[1];高鹏[1];宋佳眙[1];常伟荣[1];胡可[1];张旭辉[1];安方玉[1];颜春鲁[1,2,3];耿广琴[1];袁万英[4];赵亚娜[5]

第一作者：王芳

机构：[1]甘肃中医药大学,兰州730000;[2]甘肃省中医药研究中心,兰州730000;[3]甘肃省高校中(藏)药化学与质量研究省级重点实验室,兰州730000;[4]长春中医药大学附属医院定西医院,甘肃定西743099;[5]天水市卫生学校,甘肃天水741000

第一机构：甘肃中医药大学

年份：2025

卷号：42

期号：24

起止页码：4277

中文期刊名：中国现代应用药学

外文期刊名：Chinese Journal of Modern Applied Pharmacy

收录：;北大核心:【北大核心2023】；

基金：国家自然科学基金项目(82560967);甘肃省中医药科研课题(GZKG-2024-88);定西市科技计划项目(DX2024BY016);甘肃省高校中(藏)药化学与质量研究省级重点实验室开放基金项目(zzy-2024-02);甘肃省中医药研究中心开放课题(zyzx-2024-zx16);甘肃省高等教育教学改革研究项目(GJJGB228);甘肃中医药大学教学研究与改革项目(YBXM-202332)。

语种：中文

中文关键词：补肾活血复方藤黄健骨胶囊;绝经后骨质疏松;Sirt1/FoxO3a信号轴;自噬

外文关键词：Bushen Huoxue Fufang Tenghuang Jiangu Capsule;postmenopausal osteoporosis;Sirt1/FoxO3a signal axis;autophagy

摘要：目的观察补肾活血复方藤黄健骨胶囊对绝经后骨质疏松模型大鼠Sirt1、FoxO3a表达及自噬分子P62、Beclin1、LC3-Ⅱ表达水平变化的影响,旨在探讨补肾活血复方藤黄健骨胶囊治疗绝经后骨质疏松的作用机制。方法建立去卵巢诱导的大鼠绝经后骨质疏松模型,观察一般状况;显微CT观察股骨骨微结构变化及骨体积分数(BV/TV)、骨小梁厚度(Tb.Th);双能X线吸收仪检测骨密度;ELISA法检测ALP、CTX-Ⅰ和BGP含量;实时荧光定量PCR检测Sirt1、FoxO3a、P62的基因水平;蛋白印迹法检测Sirt1、FoxO3a、P62、Beclin1、LC3-Ⅱ的表达。结果一般状况和体质量结果显示,补肾活血复方藤黄健骨胶囊高剂量组能显著改善大鼠的一般状况,显著减轻大鼠的体质量(P<0.05)。显微CT结果显示,补肾活血复方藤黄健骨胶囊能显著减轻股骨骨微结构损伤程度,中、高剂量组能显著升高BV/TV(P<0.05或P<0.01),高剂量组能显著升高Tb.Th(P<0.01)。双能X线吸收仪检测结果显示,补肾活血复方藤黄健骨胶囊各剂量组显著升高骨密度(P<0.01)。ELISA结果显示,补肾活血复方藤黄健骨胶囊各剂量组显著升高ALP含量(P<0.01),中、高剂量组显著升高BGP含量(P<0.01),高剂量组能显著降低CTX-Ⅰ含量(P<0.01)。Sirt1/FoxO3信号轴关键分子基因和蛋白检测结果显示补肾活血复方藤黄健骨胶囊各剂量组能显著升高Sirt1/FoxO3a信号轴关键分子Sirt1、FoxO3a基因表达和蛋白表达(P<0.05或P<0.01)。自噬分子基因和蛋白检测结果显示补肾活血复方藤黄健骨胶囊各剂量组能显著降低自噬分子P62基因表达和蛋白表达(P<0.01),显著升高自噬分子Beclin1蛋白表达(P<0.05或P<0.01),中、高剂量组能显著升高自噬分子LC3-Ⅱ蛋白表达(P<0.01)。结论补肾活血复方藤黄健骨胶囊能够减轻股骨骨微结构损伤,治疗绝经后骨质疏松,可能是通过激活Sirt1/FoxO3a信号轴关键分子Sirt1、FoxO3a表达,进一步通过下调自噬分子P62和上调自噬分子Beclin1、LC3-Ⅱ来实现的。
OBJECTIVE To observe the effects of Bushen Huoxue Fufang Tenghuang Jiangu Capsule on the expression levels of Sirt1,FoxO3a,and the expression levels of autophagy molecules P62,Beclin1,and LC3-Ⅱ,so as to investigate the mechanism of Bushen Huoxue Fufang Tenghuang Jiangu Capsule in postmenopausal osteoporosis rats.METHODS The rats postmenopausal osteoporosis model was established using bilateral ovariectomy method,the general situation was observed.The microstructure changes were observed,and the bone volume fraction(BV/TV),the trabecular thickness(Tb.Th)of femoral bone was checked using micro CT.The ALP,CTX-Ⅰ and BGP content were detected by ELISA method.The gene levels of Sirt1,FoxO3a,and P62 were detected using real time fluorescence quantitative PCR.The expression of Sirt1,FoxO3a,P62,Beclin1,LC3-Ⅱ was analyzed using Western blotting.RESULTS The general situation and weight results showed that the general situation significantly improved,the weight significantly reduced in the high-dose group of Bushen Huoxue Fufang Tenghuang Jiangu Capsule(P<0.05).The micro CT results showed that the Bushen Huoxue Fufang Tenghuang Jiangu Capsule could significantly reduce the degree of femoral bone microstructure damage,the medium-dose and high-dose groups of the Bushen Huoxue Fufang Tenghuang Jiangu Capsule could significantly increase BV/TV(P<0.05 or P<0.01),while the high-dose group could significantly increase Tb.Th(P<0.01).The detection results of dual energy X-ray absorptiometry showed that the Bushen Huoxue Fufang Tenghuang Jiangu Capsule could significantly increase bone mineral density(P<0.01).The ELISA results showed that the ALP contents significantly increased in the each-dose group of Bushen Huoxue Fufang Tenghuang Jiangu Capsule(P<0.01),the BGP contents significantly increased in the the medium-dose and high-dose groups of Bushen Huoxue Fufang Tenghuang Jiangu Capsule(P<0.01),the CTX-Ⅰ contents significantly decreased in the high-dose group of Bushen Huoxue Fufang Tenghuang Jiangu Capsule(P<0.01).The detection results of gene and protein of key molecules in signal axis showed that the gene and protein expressions of key molecule Sirt1,FoxO3a in the Sirt1/FoxO3a signal axis significantly raised in the each-dose group of Bushen Huoxue Fufang Tenghuang Jiangu Capsule(P<0.05 or P<0.01).The detection results of gene and protein of autophagy molecule showed that each-dose group of Bushen Huoxue Fufang Tenghuang Jiangu Capsule could significantly reduce the gene and protein expression of autophagy molecule P62(P<0.01),and significantly increase the protein expression of autophagy molecule Beclin1(P<0.05 or P<0.01),the medium-dose and high-dose groups could significantly increase the protein expression of autophagy molecule LC3-Ⅱ(P<0.01).CONCLUSION Bushen Huoxue Fufang Tenghuang Jiangu Capsule can alleviate microstructural damage to femoral bones and treat postmenopausal osteoporosis,possibly through activating the Sirt1 and FoxO3a expression of key molecules in Sirt1/FoxO3a signal axis,and further down-regulating the expression level of autophagy molecule P62 and upregulating the expression levels of autophagy molecules Beclin1 and LC3-Ⅱ.