文献类型：期刊文献

中文题名：西部地区濒危药用植物秦艽遗传多样性的RAPD分析

英文题名：RAPD analyses of genetic diversity of endangered G. macrophylla Pall. in western China

作者：侯茜[1];雷英[1];刘丽莎[1];吴迪[1]

第一作者：侯茜

机构：[1]甘肃中医学院

第一机构：甘肃中医药大学

年份：2013

卷号：28

期号：1

起止页码：214

中文期刊名：中华中医药杂志

外文期刊名：China Journal of Traditional Chinese Medicine and Pharmacy

收录：CSTPCD;;北大核心:【北大核心2011】；CSCD:【CSCD_E2013_2014】；

基金：国家中医药管理局项目(No.04-05ZL23);甘肃省教育厅项目(No.0606B-01)~~

语种：中文

中文关键词：秦艽;RAPD;遗传多样性

外文关键词：Gentiana macrophylla Pall.; RAPD; Genetic diversity

摘要：目的:对甘肃、陕西、宁夏、四川及青海5个主产地秦艽的遗传多样性进行分析。方法:用随机扩增多态性DNA标记(RAPD)技术对11个秦艽种群90个个体基因组DNA的遗传多样性进行检测。结果:RAPD 8条引物检测到65条扩增条带,其中有59条是多态的,多态位点百分比达到97.78%。通过聚类分析可将秦艽的11个种群聚为两类,四川和陕西两省4个种群聚为一类;甘肃、宁夏和青海省7个种群聚为一类。结论:甘肃省环县种群、子午岭种群和华亭种群应作为秦艽的重要种群加以优先重点保护。
Objective：The study analyzed the genetic diversity of G.macrophylla Pall.in its native growth places in Gansu,Shanxi,Ningxia,Sichuan and Qinghai provinces.Methods： Detect the genetic diversity of the DNA of 90 G.macrophylla Palls from 11 populations through RAPD technique.Results： 65 expansion bands with 59 being polymorphism were detected through 8 primers.Polymorphism rate is 97.78%.The 11 populations were classified into 2 groups through classification analyses,with 4 populations from Sichuan and Shanxi provinces into one group,and 7 populations from Ganshu,Ningxia and Qinghai provinces into another group.Conclusion： The populations in Huan,Ziwuling,and Huating Counties of Gansu Province should be protected with priority as the important populations.