文献类型：期刊文献

中文题名：热补针法对类风湿关节炎家兔关节滑膜LDH、SDH和CCO活性的影响

英文题名：Effect of Heat Reinforcing Acupuncture Manipulation on Articular Synovium LDH,SDH and CCO in Rheumatoid Arthritis Rabbits

作者：杜小正[1];王金海[2];秦晓光[1];方晓丽[1];张小江[1]

第一作者：杜小正

机构：[1]甘肃中医药大学,兰州730000;[2]兰州大学第二医院,兰州730000

第一机构：甘肃中医药大学

年份：2016

卷号：35

期号：10

起止页码：1256

中文期刊名：上海针灸杂志

外文期刊名：Shanghai Journal of Acupuncture and Moxibustion

收录：CSTPCD

基金：甘肃省教育厅第二批科研项目(1106B-08);甘肃中医药大学中青年科研基金项目(gszyxy2010-4)

语种：中文

中文关键词：针刺补法;关节炎,类风湿;乳酸脱氢酶;琥珀酸脱氢酶;细胞色素氧化酶

外文关键词：Acupuncture reinforcing method; Arthritis; rheumatoid; Lactate dehydrogenase; Succinate dehydrogenase; Cytochrome oxidase

摘要：目的观察热补针法对类风湿关节炎(RA)机体能量代谢酶的调节作用,初步解析热补针法"取热"的作用机制。方法将40只青紫蓝家兔随机分为正常组、模型组、平补平泻组、捻转补法组和热补针法组。除正常组外,采用卵蛋白诱导法和低温冷冻法对其他4组家兔进行RA寒证模型复制。造模成功后第2天,正常组和模型组与其他针刺组相同方法抓取与固定(捆绑)1次/d,30 min/次。平补平泻组施以平补平泻法,捻转补法组施以捻转补法,热补针法组施以热补法,每次操作1 min,留针30 min/次,1次/d,共7 d。观察治疗前后RA家兔膝关节周径和关节滑膜组织病理切片炎症积分的变化;干预结束后处死家兔,快速分离关节滑膜,冰冻切片,借助组织化学染色技术观察膝关节滑膜组织乳酸脱氢酶(LDH)、琥珀酸脱氢酶(SDH)和细胞色素氧化酶(CCO)活性改变。结果针刺干预后,平补平泻组、捻转补法组、热补针法组都能减少RA家兔膝关节周径,但热补针法组对减少RA家兔的膝关节周径作用优于平补平泻组和捻转补法组(P<0.05);热补针法组对减少RA家兔的关节滑膜组织病理切片炎症积分方面,也优于平补平泻组和捻转补法组。模型组滑膜LDH积分光度、阳性总面积、面积百分比显著高于正常组(P<0.05);平补平泻组、捻转补法组和热补针法组滑膜LDH积分光度、阳性总面积、面积百分比均显著低于模型组(P<0.05);热补针法组LDH积分光度、阳性总面积、面积百分比均显著低于平补平泻组和捻转补法组(P<0.05)。与正常组比较,模型组家兔关节滑膜SDH、CCO活性显著增高(P<0.05)。平补平泻组、捻转补法组和热补针法组家兔滑膜SDH和CCO积分光度、阳性总面积、面积百分比均显著高于模型组(P<0.05);热补针法组SDH和CCO积分光度、阳性总面积、面积百分比显著高于平补平泻组和捻转补法组(P<0.05)。结论热补针法治疗RA疗效确切,可增强RA模型家兔SDH、CCO活性,从而增强有氧代谢,使机体局部产生更多的能量,这可能是热补针法"取热"的可能机制之一。
Objective To investigate the regulating effect of heat reinforcing acupuncture manipulation on body energy metabolism enzymes in rheumatoid arthritis（RA） and preliminarily explain the mechanism of heat-producing action of heat reinforcing acupuncture manipulation.Method Forty chinchilla rabbits were randomized into normal,model,equal reinforcement and reduction,twirling reinforcement,and heat reinforcing acupuncture manipulation groups.A model of cold syndrome-type RA was made by ovalbumin induction and exposure to low temperature in the other four groups not including the normal group.From two days after successful model making,the normal and model groups were grabbed and fastened（bound） by the same way as for the acupuncture groups,30 min once daily.The equal reinforcement and reduction group received even reinforcing-reducing method;the twirling reinforcement group,twirling reinforcement method;the heat reinforcing group,heat reinforcing acupuncture manipulation.The needle was manipulated for 1 min and retained for 30 min once daily,for a total of seven days.The RA rabbit knee joint circumference was measured and the inflammation score was recorded according to synovial histopathological sections before and after treatment.After the completion of intervention,the rabbits were sacrificed and the articular synovium was rapidly separated for frozen sections.Articular synovium lactate dehydrogenase（LDH）,succinate dehydrogenase（SDH） and cytochrome oxidase（CCO） activities were measured by histochemical staining.Result After acupuncture intervention,the RA rabbit knee joint circumference was shortened in all the equal reinforcement and reduction,twirling reinforcement,and heat reinforcing acupuncture manipulation groups,but the shortening effect on the RA rabbit knee joint circumference was better in the heat reinforcing acupuncture manipulation group than in the equal reinforcement and reduction and twirling reinforcement method groups（P〈0.05）;the inflammation score recorded according to the RA rabbit synovial histopathological sections was also decreased more in the heat reinforcing acupuncture manipulation group than in the equal reinforcement and reduction and twirling reinforcement method groups（P〈0.05）.Synovial LDH integral optical density,total positive area and area percentage were significantly higher in the model group than in the normal group（P〈0.05）,and significantly lower in the equal reinforcement and reduction,twirling reinforcement,and heat reinforcing acupuncture manipulation groups than in the model group（P〈0.05） and also in the heat reinforcing acupuncture manipulation group than in the equal reinforcement and reduction and twirling reinforcement method groups（P〈0.05）.Rabbit synovial SDH and CCO activities were significantly higher in the model group than in the normal group（P〈0.05）.Rabbit synovial SDH and CCO integral optical density,total positive area and area percentage were significantly higher in the equal reinforcement and reduction,twirling reinforcement,and heat reinforcing acupuncture manipulation groups than in the model group（P〈0.05） and also in the heat reinforcing acupuncture manipulation group than in the equal reinforcement and reduction and twirling reinforcement method groups（P〈0.05）.Conclusion Heat reinforcing acupuncture manipulation has a definite therapeutic effect on RA and can increase SDH and CCO activities to enhance aerobic metabolism and produce more local energy in a rabbit model of RA.That may be the mechanism by which heat reinforcing acupuncture manipulation produces heat.