文献类型：期刊文献

中文题名：油橄榄叶抗糖尿病活性部位筛选

英文题名：Screening for anti-diabetic effective fraction in leaves of Olea europaea

作者：张佳[1,2];卜令娜[2,3];裴栋[2];刘晔玮[3];任远[1];邸多隆[1,2]

第一作者：张佳

机构：[1]甘肃中医学院药学系甘肃省中药药理与毒理学重点实验室,甘肃兰州730000;[2]中国科学院兰州化学物理研究所中国科学院西北特色植物资源化学重点实验室和甘肃省天然药物重点实验室,甘肃兰州730000;[3]兰州大学公共卫生学院营养与食品卫生研究所,甘肃兰州730000

第一机构：甘肃中医药大学药学院（西北中藏药协同创新中心办公室）|甘肃中医药大学科研实验中心（甘肃省中医药标准化技术委员会秘书处）

年份：2013

卷号：44

期号：13

起止页码：1807

中文期刊名：中草药

外文期刊名：Chinese Traditional and Herbal Drugs

收录：CSTPCD;;Scopus;北大核心:【北大核心2011】；CSCD:【CSCD2013_2014】；

基金：中国科学院"百人计划"资助项目(2007-0629141051);国家自然科学基金资助项目(20974116)

语种：中文

中文关键词：油橄榄叶;抗糖尿病活性;α-淀粉酶;蛋白质非酶糖基化;四氧嘧啶;糖尿病模型

外文关键词：leaves of Olea europaea; anti-diabetic activity; a-amylase; non-enzymatic glycosylation of protein; alloxan; diabetic model

摘要：目的筛选油橄榄叶抗糖尿病活性部位。方法应用回流提取法和大孔吸附树脂技术,从油橄榄叶中提取6个部位(A～F)。对6个部位进行α-淀粉酶抑制实验、蛋白质非酶糖基化抑制实验和四氧嘧啶致小鼠糖尿病模型实验,综合评价和筛选抗糖尿病活性部位。结果 C～F部位对α-淀粉酶、蛋白质非酶糖基化均显示明显的抑制活性,其中以D部位活性最强,且呈量效关系。C、D部位降血糖作用较明显,且以D部位活性最显著。结论油橄榄叶具有潜在抗糖尿病活性,其中D部位的活性尤为显著。
Objective To screen the anti-diabetic effective fraction in the leaves of Olea europaea. Methods Different fractions （Frs. A-F） were prepared using reflux extraction and separation with macroporous resins, α-Amylase system, non-enzymatic glycation system, and alloxan-induced diabetic models of mice were used to evaluate the anti-diabetic activities of the different fractions, respectively. Results Frs. C-F fractions showed the significant inhibitory activities against a-amylase system and non-enzymatic glycation system. Especially, fraction D showed the best anti-diabetic activity compared with the other fractions, which also displayed a good dose-effect relationship. The anti-diabetic activities of Frs. C and D were both obvious and Fr. D showed more significance. Conclusion The leaves of O. europaea show the potential anti-diabetic activity and the most effective fraction is ascribed to Fr. D.