文献类型：期刊文献

英文题名：Characteristics and immune dynamics of peripheral blood immune cells and cytokines in individuals with chronic hepatitis B virus infection

作者：Xiao, Lixin[1];Dong, Zhuanli[1];He, Yao[2];Fan, Jingchun[3];Duan, Huichun[4];Jiang, Xiaomei[5];Wei, Changhao[6];Ma, Tingting[2];Zhang, Yi[7,8]

第一作者：Xiao, Lixin

通信作者：Dong, ZL[1]

机构：[1]Gansu Univ Chinese Med, Lanzhou Petrochem Gen Hosp, Affiliated Hosp 4, Dept Qual Management, Lanzhou 730060, Gansu, Peoples R China;[2]Gansu Univ Chinese Med, Sch Publ Hlth, Lanzhou 730030, Gansu, Peoples R China;[3]Gansu Univ Chinese Med, Ctr Evidence Based Med, Sch Publ Hlth, Lanzhou 730030, Gansu, Peoples R China;[4]Gansu Univ Chinese Med, Lanzhou Petrochem Gen Hosp, Affiliated Hosp 4, Dept Gastroenterol, Lanzhou 730060, Gansu, Peoples R China;[5]Gansu Univ Chinese Med, Lanzhou Petrochem Gen Hosp, Affiliated Hosp 4, Dept Psychosomat & Sleep Med, Lanzhou 730060, Gansu, Peoples R China;[6]Gansu Prov Hosp Chinese Med, Dept Orthoped Med, Lanzhou 730050, Gansu, Peoples R China;[7]Gansu Univ Chinese Med, Clin Coll Chinese Med, Lanzhou 730030, Gansu, Peoples R China;[8]Gansu Univ Chinese Med, Lanzhou Petrochem Gen Hosp, Affiliated Hosp 4, Lanzhou 730060, Gansu, Peoples R China

第一机构：甘肃中医药大学

通信机构：[1]corresponding author), Gansu Univ Chinese Med, Lanzhou Petrochem Gen Hosp, Affiliated Hosp 4, Dept Qual Management, Lanzhou 730060, Gansu, Peoples R China.|[10735]甘肃中医药大学;

年份：2026

卷号：16

期号：1

外文期刊名：SCIENTIFIC REPORTS

收录：;Scopus(收录号：2-s2.0-105041183667);WOS:【SCI-EXPANDED(收录号:WOS:001788059000001)】；

基金：This work was supported by grants from the Key R&D Program of Gansu Province, China (Grant No. 25YFFA044); the Gansu Provincial Higher Education Institutions Young Doctoral Support Project, China (Grant No. 20250B-064); the Open Project of the Key Laboratory of Dunhuang Medicine and Transformation, Ministry of Education, China (Grant No. DHYX24-17); the 12th Batch of Provincial Science and Technology Plan (Joint Research Fund) Project of Gansu Province, China (Grant No. 23JRRA1528); and the Innovation Base and Talent Plan Fund Project of Gansu Province, China (Grant No. 21JR11RA191); 2025 Lanzhou Municipal Science and Technology Development Guiding Program (Grant No. 2025-5-150).

语种：英文

外文关键词：Hepatitis B; Chronic courses; Cytokines; Immune cells; Systematic

摘要：Hepatitis B virus (HBV)-related immune injury-mediated local liver inflammation and hepatocyte death are critical for the progression of chronic hepatitis B (CHB). This cross-sectional study aimed to characterize the systematic variations of peripheral blood immune cells and the corresponding changes of cytokines/chemokines during CHB progression, without implying causal relationships. Peripheral blood immune cell subsets were detected by multiparametric flow cytometry, and the levels of cytokines/chemokines were measured using the Luminex discovery assay. The study included CHB patients at different stages and healthy controls (HC), with data compared between groups, A multivariate analysis of variance (ANOVA) was used to adjust for potential confounding factors including age and gender. Compared with HC, initial CHB patients had a significantly higher frequency of total B cells (p < 0.001) but a lower proportion of CD4 + T cells (p < 0.01). The level of C-X-C chemokine ligand 10 (CXCL10) was markedly elevated in CHB patients (p < 0.01) and positively correlated with B cell frequency. Notably, CHB patients with serum Hepatitis B Surface Antigen (HBsAg) < 1500 ng/mL had higher CXCL10 levels than those with HBsAg >= 1500 ng/mL (p < 0.05). In late-stage HBV infection (HBV-LC and HBV-HCC), the frequency of NK cells was significantly lower than that in CHB patients and healthy controls, while the frequency of T cells showed a relative increase. Additionally, compared with CHB patients and HC, late-stage patients showed significantly higher levels of key cytokines/chemokines, including interferon-gamma (IFN-gamma), interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-alpha), C-C chemokine ligand 2 (CCL2), and tumor necrosis factor receptor I (TNFRI). This cross-sectional study reveals distinct alterations in peripheral blood immune cells and cytokines/chemokines across CHB progression, with CXCL10, NK cells, and multiple proinflammatory factors closely associated with disease stage and HBsAg levels. These findings may provide potential biomarkers for monitoring CHB progression and theoretical support for immune-based therapeutic strategies, but causal relationships require verification by subsequent longitudinal studies.