文献类型：期刊文献

英文题名：Screening of low-toxic zinc oxide nanomaterials and study the apoptosis mechanism of NSC-34 cells

作者：Li, Yuanyuan[1];Lu, Yan[2];Li, Jingjing[3];Li, Mei[1];Gou, Huitian[1];Sun, Xiaolin[1];Xu, Xiaoli[1];Song, Beibei[1];Li, Zhiyu[1];Ma, Yonghua[1]

第一作者：Li, Yuanyuan

通信作者：Ma, YH[1]

机构：[1]Gansu Agr Univ, Coll Vet Med, Lanzhou, Gansu, Peoples R China;[2]Northwest Normal Univ, Coll Phys & Elect Engn, Key Lab Atom & Mol Phys & Funct Mat Gansu Prov, Lanzhou, Peoples R China;[3]Gansu Univ Tradit Chinese Med, Coll Pharm, Lanzhou, Gansu, Peoples R China

第一机构：Gansu Agr Univ, Coll Vet Med, Lanzhou, Gansu, Peoples R China

通信机构：[1]corresponding author), Gansu Agr Univ, Coll Vet Med, Lanzhou, Gansu, Peoples R China.

年份：2024

卷号：19

期号：2

外文期刊名：BIOTECHNOLOGY JOURNAL

收录：;EI(收录号：20240915649264);Scopus(收录号：2-s2.0-85185883619);WOS:【SCI-EXPANDED(收录号:WOS:001177472800001)】；

基金：Gansu Agricultural University, Grant/Award Numbers: GAU-XKJS-2018-073, GAU-KYQD-2017RCZX-11, GAU-QDFC-2020-11; Lanzhou Science and Technology Plan Project, Grant/Award Numbers: 2022-2-112,2019-1-32; Gansu Province Natural Sciences Fund, Grant/Award Numbers: 18JR3RA16, 21JR1RA233; National Natural Science Foundation Project (CN),Grant/AwardNumbers:31460659,31460659, 32060822, 11964035; Key Research and Development Projects in Gansu Province, Grant/Award Number:18YF1FA080; Biotechnology Project of Gansu Agriculture and Animal Husbandry Department, Grant/Award Number:GNSW-2014-9; Open Fund of State Key Laboratory of Pathogenic Biology of Livestock Epidemic Diseases, Grant/Award Number: SKLVEB2014KFKT002; Scientific Research Project of Gansu Province, Grant/Award Number:21JR1RA233;Foundations of Northwest Normal University, Grant/AwardNumber:NWNU-LKQN2021-26

语种：英文

外文关键词：apoptosis; low-toxicity zinc oxide nanomaterials; the safety exposure limit; ZnO nanorod flowers

摘要：With the increasing application of ZnO nanomaterials (ZnO-NMts) in the biomedical field, it is crucial to assess their potential risks to humans and the environment. Therefore, this study aimed to screen for ZnO-NMts with low toxicity and establish safe exposure limits, and investigate their mechanisms of action. The study synthesized 0D ZnO nanoparticles (ZnO NPs) and 3D ZnO nanoflowers (ZnO Nfs) with different morphologies using a hydrothermal approach for comparative research. The ZnO-NMts were characterized using X-ray diffraction (XRD), scanning electron microscopy (SEM), and transmission electron microscopy (TEM). Mouse brain neuronal cells (NSC-34) were incubated with ZnO NMts for 6, 12, and 24 h, and the cell morphology was observed using TEM. The toxic effects of ZnO Nfs on NSC-34 cells were studied using CCK-8 cell viability detection, reactive oxygen species (ROS) measurement, caspase-3 activity detection, Annexin V-FITC/PI apoptosis assay, and mitochondrial membrane potential (Delta phi m) measurement. The results of the research showed that ZnO-NMts caused cytoplasmic vacuolization and nuclear pyknosis. After incubating cells with 12.5 mu g mL(-1) ZnO-NMts for 12 h, ZnO NRfs exhibited the least toxicity and ROS levels. Additionally, there was a significant increase in caspase-3 activity, depolarization of mitochondrial membrane potential (Delta phi m), and the highest rate of early apoptosis.This study successfully identified ZnO NRfs with the lowest toxicity and determined the safe exposure limit to be < 12.5 mu g mL(-1) (12 h). These findings will contribute to the clinical use of ZnO NRfs with low toxicity and provide a foundation for further research on their potential applications in brain disease treatment.