文献类型：期刊文献

中文题名：红芪多糖对db/db小鼠糖尿病心肌病的病理形态与胶原表达的影响

英文题名：Effect of Radix hedysari polysaccharide on pathological morphology of diabetic cardiomyopathy and expression of collagen in db/db mice

作者：张花治[1];金智生[1];王东旭[1];和彩玲[1];何流[1];贾龙[1];楚惠媛[1];邵晶[1]

第一作者：张花治

机构：[1]甘肃中医药大学中医临床学院,兰州730000

第一机构：甘肃中医药大学中医临床学院

年份：2018

卷号：34

期号：16

起止页码：1971

中文期刊名：中国临床药理学杂志

外文期刊名：The Chinese Journal of Clinical Pharmacology

收录：CSTPCD;;北大核心:【北大核心2017】；CSCD:【CSCD2017_2018】；

基金：国家自然科学基金地区科学基金资助项目(81360538);甘肃省青年科技基金资助项目(145RJYA297)

语种：中文

中文关键词：糖尿病心肌病;红芪多糖;病理形态;Ⅰ型胶原蛋白;Ⅲ型胶原蛋白

外文关键词：diabetic cardiomyopathy;Hedysarum polybotrys polysacchcaide;pathological morphology;collagen Ⅰ;collagen Ⅲ

摘要：目的研究红芪多糖(HPS)对db/db小鼠糖尿病心肌病的病理形态及Ⅰ、Ⅲ型胶原蛋白表达的影响。方法用随机数表法将6周龄雄性db/db小鼠分为5组:正常组、模型组、对照组和高、中、低3个剂量实验组。模型组和正常组ab/m小鼠均给予0.9%Na Cl 10μL·g-1灌胃;对照组给予4 mg·kg-1·d-1罗格列酮混悬液灌胃;实验组分别给予200,100,50 mg·kg-1·d-1HPS混悬液灌胃,每组12只,均连续灌胃8周。Masson染色观察心肌组织病理改变,实时荧光定量核酸扩增法与蛋白质印迹法分别检测心肌组织Ⅰ、Ⅲ型胶原α与mRNA与蛋白的表达。结果模型组、高、中2个剂量实验组和对照组的Ⅰ型胶原的mRNA分别为5.04±1.35,1.57±0.21,2.73±0.57,2.06±0.47;这4组的Ⅲ型胶原的mRNA分别为3.12±0.25,1.59±0.09,1.95±0.12,1.67±0.21;这4组的Ⅰ型胶原蛋白表达分别为1.29±0.05,0.87±0.03,0.96±0.04,0.89±0.02;这4组的Ⅲ型胶原蛋白表达分别为0.89±0.03,0.69±0.01,0.76±0.01,0.69±0.01。3个给药组与模型组比较,上述指标均明显降低,差异均有统计学意义(均P<0.01;除了中剂量实验组的Ⅰ型胶原的mRNA为P<0.05以外)。心肌组织中Ⅰ、Ⅲ型胶原的蛋白表达与Masson染色胶原纤维阳性染色面积值呈正相关,相关系数分别为0.955,0.909(P<0.01)。结论 HPS能抑制Ⅰ、Ⅲ型胶原的表达水平及Ⅰ/Ⅲ胶原蛋白的比值,其对糖尿病心肌病db/db小鼠的心肌保护作用可能是通过影响Ⅰ、Ⅲ型胶原的表达而发挥作用。
Objective To investigate the effect of Radix hedysari polysaccharide（ HPS） on the myocardial pathological morphology of diabetic cardiomyopathy（DCM） and expression of collagen type Ⅰ and Ⅲ in db/db mice. Methods The six-week-old male db/db mice were randomly divided into five groups,each group had 12 mice： experimental-L,experimental-M,experimental-H group（ HPS： 200,100,50 mg·kg^-1）,control group（ rosiglitazone： 4 mg·kg^-1） and model group（ 0.9% NaCl,10 μL·g^-1 gavege）,while 12 db/m mice（ 6-week-old） were normal group. Changes of pathologic morphology in myocardium of diabetic cardiomyopathy（ DCM） mice was investigated with Masson staining. The q PCR and Western blotting were used to detect the expression of collagen Ⅰand Ⅲ MRA and protein respectively inmyocardium. Results The expression of typeⅠcollagen mRNA in model group,experimental-M group,experimental-H group,control group were 5.04±1.35,1.57±0.21,2.73±0.57,2.06±0.47; the type Ⅲ collagen mRNA in the 4 groups were 3.12±0.25,1.59±0.09,1.95±0.12,1.67±0.21; the expression of type Ⅰ collagen protein in the 4 groups were 1.29±0.05,0.87±0.03,0.96±0.04,0.89±0.02; the expression of type Ⅲ collagen protein in the 4 groups were 0.89±0.03,0.69±0.01,0.76±0.01,0.69 ± 0.01. Comparison between 3 drug groups with the model group,the difference of the factors were significantly lower（ all P〈0.01; except for the experimental-M group of typeⅠ collagen mRNA,P〈0.05）. The expression of Ⅰ and type Ⅲ collagen in myocardium was positively correlated with the value of Masson staining and the positive staining area of collagen fibers,and the correlation coefficients were 0. 955 and 0. 909（ P〈0.01）. Conclusion HPS can inhibit the expression level of Collagen Ⅰ,Collagen Ⅲ and ratio of theⅠ/Ⅲ collagen,myocardial protective effects on diabetic cardiomyopathy may be mediated by the effects of type Ⅰand type Ⅲ collagen expression.