文献类型：期刊文献

中文题名：兰州百合多糖铁(Ⅲ)配合物制备工艺的Box-Behnken响应面法优化及其体外抗氧化活性评价

英文题名：Optimization of Lanzhou Lily Polysaccharide-Fe(Ⅲ)Complex Preparation using Box-behnken Design and Evaluation of its in Vitro Antioxidant Activity

作者：李小军[1];马晓辉[1,2];段国建[1];姜红[3];曾凡逵[3];高作旺[4];董文静[5];王引权[1,2];晋玲[1,2]

第一作者：李小军

机构：[1]甘肃中医药大学药学院,甘肃兰州730000;[2]西北中藏药省部共建协同创新中心,甘肃兰州730000;[3]中国科学院兰州化学物理研究所,甘肃兰州730000;[4]兰州甜甜百合有限公司,甘肃兰州730000;[5]甘肃科技馆,甘肃兰州730070

第一机构：甘肃中医药大学药学院（西北中藏药协同创新中心办公室）

年份：2024

卷号：40

期号：12

起止页码：201

中文期刊名：现代食品科技

外文期刊名：Modern Food Science and Technology

收录：;北大核心:【北大核心2023】；

基金：道地药材生态种植及质量保障项目(国中医药科技〔2020〕153号);财政部和农业农村部:国家现代农业产业技术体系资助(CARS-21);西北中藏药省部共建协同创新中心项目(Xbzzy-2022-01);甘肃省科技重大专项(23ZDFA013-1)。

语种：中文

中文关键词：兰州百合;多糖铁(Ⅲ)配合物;Box-Behnken响应面法;工艺优化;抗氧化能力

外文关键词：Lilium davidii var.willmottiae(E.H.Wilson)Raffill;polysaccharide-Fe(Ⅲ)complex;Box-Behnken response surface methodology;process optimization;antioxidant capacity

摘要：该研究以兰州百合多糖和FeCl_(3)为原料,以Fe^(3+)含量为评价指标,采用单因素实验及Box-Behnken响应面法优化兰州百合多糖铁(Ⅲ)配合物制备工艺,获得最佳参数;采用红外光谱(Infrared Spectroscopy,IR)和紫外可见光光谱法(Ultraviolet-Visible Spectroscopy,UV)进行其结构分析,通过对DPPH自由基和ABTS^(+)自由基清除能力评价兰州百合多糖铁(Ⅲ)配合物体外抗氧化活性。结果表明,兰州百合多糖铁(Ⅲ)配合物的制备最佳工艺为反应温度61.10℃、pH值6.92、反应时间1.38 h,在此反应条件下,兰州百合多糖铁(Ⅲ)配合物Fe^(3+)含量为7.32%;IR和UV分析表明说明兰州百合多糖成功与Fe^(3+)螯合;兰州百合多糖铁(Ⅲ)配合物和兰州百合多糖清除DPPH自由基的半抑制浓度(Half-maximal Inhibitory Concentrations,IC_(50))分别是0.63 mg/mL和0.79 mg/mL,清除ABTS^(+)自由基的IC_(50)分别是0.71 mg/mL和0.76 mg/mL。该研究成功制备了具有抗氧化活性的兰州百合多糖铁(Ⅲ)配合物,为兰州百合资源的高值化利用和大健康新产品开发提供了一定的理论依据。
Lanzhou lily polysaccharides and ferric chloride were used as raw materials to prepare the Lanzhou lily polysaccharide-Fe(Ⅲ)complex.The preparation conditions were optimized using the Fe^(3+)content as the evaluation index based on a single-factor experimental design and Box-Behnken response surface methodology.The structure of the polysaccharide-Fe(Ⅲ)complex was analyzed using infrared spectroscopy and ultraviolet-visible spectroscopy,and its in vitro antioxidant activity was evaluated based on the DPPH and ABTS^(+)radical scavenging abilities.The results showed that the optimal parameters for the preparing Lanzhou lily polysaccharide-Fe(Ⅲ)complex were as follows:reaction temperature of 61.10℃,pH of 6.92,and reaction time of 1.38 h.The Fe^(3+)content of the complex was 7.32%under these reaction conditions.The results of infrared and ultraviolet analyses indicated that Lanzhou lily polysaccharides chelated with Fe^(3+).Furthermore,the half-maximal inhibitory concentrations of the Lanzhou lily polysaccharide-Fe(Ⅲ)complex and Lanzhou lily polysaccharides against DPPH radicals were 0.63 and 0.79 mg/mL,respectively.The corresponding half-maximal inhibitory concentrations against ABTS^(+)radicals were 0.71 and 0.76 mg/mL,respectively.Additionally,the antioxidant activity of the complex was considerably stronger than that of lily polysaccharides.The Lanzhou lily polysaccharide-Fe(Ⅲ)complex with antioxidant activity was successfully prepared.The findings of this study provide a theoretical foundation for maximizing the use of Lanzhou lily resources and creating new health products.