文献类型：期刊文献

英文题名：Analysis of the potential biological value of pyruvate dehydrogenase E1 subunit β in human cancer

作者：Rong, Yao[1,2,3,4];Liu, Song-Hua[1,2];Tang, Ming-Zheng[1,2,3,4];Wu, Zhi-Hang[1];Ma, Guo-Rong[1];Li, Xiao-Feng[1];Cai, Hui[2,3,4]

第一作者：Rong, Yao

通信作者：Cai, H[1]

机构：[1]Gansu Univ Chinese Med, Clin Med Coll 1, Lanzhou 730000, Gansu, Peoples R China;[2]Gansu Prov Hosp, Gen Surg Clin Med Ctr, 204 Donggang West Rd, Lanzhou 730000, Gansu, Peoples R China;[3]Gansu Prov Hosp, Key Lab Mol Diagnost & Precis Med Surg Oncol Gansu, Lanzhou 730000, Gansu, Peoples R China;[4]Gansu Prov Hosp, NHC Key Lab Diag & Therapy Gastrointestinal Tumor, Lanzhou 730000, Gansu, Peoples R China

第一机构：甘肃中医药大学

通信机构：[1]corresponding author), Gansu Prov Hosp, Gen Surg Clin Med Ctr, 204 Donggang West Rd, Lanzhou 730000, Gansu, Peoples R China.

年份：2024

卷号：16

期号：1

起止页码：144

外文期刊名：WORLD JOURNAL OF GASTROINTESTINAL ONCOLOGY

收录：;Scopus(收录号：2-s2.0-85184892205);WOS:【SCI-EXPANDED(收录号:WOS:001153912700015)】；

基金：Supported by The 2021 Central-Guided Local Science and Technology Development Fund; Lanzhou COVID-19 Prevention and Control Technology Research Project, No. 2020-XG-1; Gansu Province Outstanding Graduate Student "Innovation Star" Project, No. 2022CXZX-748, No. 2022CXZX-746.

语种：英文

外文关键词：Cuprotosis; Pyruvate dehydrogenase E1 subunit beta; Pan-cancer; Prognosis; Liver cancer

摘要：BACKGROUND The pyruvate dehydrogenase E1 subunit beta (PDHB) gene which regulates energy metabolism is located in mitochondria. However, few studies have elucidated the role and mechanism of PDHB in different cancers. AIM To comprehensive pan-cancer analysis of PDHB was performed based on bioinformatics approaches to explore its tumor diagnostic and prognostic value and tumor immune relevance in cancer. In vitro experiments were performed to examine the biological regulation of PDHB in liver cancer. METHODS Pan-cancer data related to PDHB were obtained from the Cancer Genome Atlas (TCGA) database. Analysis of the gene expression profiles of PDHB was based on TCGA and Genotype Tissue Expression Dataset databases. Cox regression analysis and Kaplan-Meier methods were used to assess the correlation between PDHB expression and survival prognosis in cancer patients. The correlation between PDHB and receiver operating characteristic diagnostic curve, clinicopathological staging, somatic mutation, tumor mutation burden (TMB), microsatellite instability (MSI), DNA methylation, and drug susceptibility in pan-cancer was also analyzed. Various algorithms were used to analyze the correlation between PDHB and immune cell infiltration and tumor chemotaxis environment, as well as the co-expression analysis of PDHB and immune checkpoint (ICP) genes. The expression and functional phenotype of PDHB in single tumor cells were studied by single-cell sequencing, and the functional enrichment analysis of PDHB-related genes was performed. The study also validated the level of mRNA or protein expression of PDHB in several cancers. Finally, in vitro experiments verified the regulatory effect of PDHB on the proliferation, migration, and invasion of liver cancer. RESULTS PDHB was significantly and differently expressed in most cancers. PDHB was significantly associated with prognosis in patients with a wide range of cancers, including kidney renal clear cell carcinoma, kidney renal papillary cell carcinoma, breast invasive carcinoma, and brain lower grade glioma. In some cancers, PDHB expression was clearly associated with gene mutations, clinicopathological stages, and expression of TMB, MSI, and ICP genes. The expression of PDHB was closely related to the infiltration of multiple immune cells in the immune microenvironment and the regulation of tumor chemotaxis environment. In addition, single-cell sequencing results showed that PDHB correlated with different biological phenotypes of multiple cancer single cells. This study further demonstrated that down-regulation of PDHB expression inhibited the proliferation, migration, and invasion functions of hepatoma cells. CONCLUSION As a member of pan-cancer, PDHB may be a novel cancer marker with potential value in diagnosing cancer, predicting prognosis, and in targeted therapy.