文献类型：期刊文献

中文题名：不同发酵培养基产凝乳酶比较研究

英文题名：Comparative studies on chymosin produced by different media

作者：安志刚[1];陈鑫[1];韩黎明[1];张卫兵[2];师希雄[2];张忠明[2]

第一作者：安志刚

机构：[1]甘肃中医药大学生化系;[2]甘肃农业大学食品科学与工程学院

第一机构：甘肃中医药大学定西校区

年份：2015

卷号：34

期号：11

起止页码：47

中文期刊名：中国酿造

外文期刊名：China Brewing

收录：CSTPCD;;北大核心:【北大核心2014】；

基金：国家自然科学基金项目(31560442);甘肃省农业科技创新项目(GNCX-2014-31)

语种：中文

中文关键词：凝乳酶;解淀粉芽孢杆菌;培养基;发酵

外文关键词：chymosin; Bacillus amyloliquefaciens; medium; fermentation

摘要：为进一步研究解淀粉芽孢杆菌(Bacillus amyloliquefaciens)D4在不同发酵培养基中的生长特性,该试验选用3种培养基进行比较。菌株D4在不同培养基中利用营养物质和代谢产物的能力不同,这种差异体现在培养基的酶活力、p H值、蛋白质含量、还原糖含量、菌体生物量的不同变化。3种培养基中,培养基Ⅱ产凝乳酶活力最高,在72 h达到最大值4 533.33 SU/m L,并且各种指标变化平稳,是实验室研究摇瓶发酵特性的首选培养基。
In order to further research the growth characteristics of Bacillus amyloliquefaciens D4 in different fermentation media, three kinds of cul- ture medium were compared in the study. The strain D4 had different abilities to use the nutrients and produced different metabolite in different cul- ture mediums. These differences were reflected in enzyme activity, pH, protein content, reducing sugar content and biomass. The chymosin activity of medium II was the highest in the three kinds of culture medium, and could reach to the maximum 4 533.33 SU/ml at 72 h, and changes of all indica- tors was steady. Medium II was the preferred medium to investigate the shake flask fermentation peculiarity.