文献类型：期刊文献

中文题名：黄芪种子发芽检验标准化研究

英文题名：Study on Standardization of Germination Test of Astragalus membranacus Seed

作者：张延红[1];李金田[1];卢有媛[1];杜弢[1]

第一作者：张延红

机构：[1]甘肃中医药大学药学院,兰州730000

第一机构：甘肃中医药大学药学院（西北中藏药协同创新中心办公室）

年份：2016

卷号：27

期号：7

起止页码：998

中文期刊名：中国药房

外文期刊名：China Pharmacy

收录：CSTPCD;;北大核心:【北大核心2014】；

基金：国家中医药管理局基金资助项目-国家基本药物所需中药材种子种苗繁育基地建设

语种：中文

中文关键词：黄芪;种子;种子休眠;发芽温度;发芽床;发芽计数时间;发芽率;发芽指数;发芽势

外文关键词：Astragalus membranaceus; Seed; Seed dormancy; Germination temperature; Germination bed; Germination counting time; Germination rate; Germination index; Germinative force

摘要：目的:确定适宜于黄芪种子萌发的最优条件,为制订黄芪种子检验规程提供依据。方法:以98%硫酸处理30 min+35℃温水浸泡9 h打破种子休眠,通过不同温度、不同发芽床和不同光照条件进行处理,以发芽率、发芽势和发芽指数3个指标对不同处理方法进行评价。结果:随着温度的升高,种子的发芽率、发芽指数和发芽势呈下降趋势;30℃处理下种子发芽率、发芽指数和发芽势显著低于其他处理,其中15℃的发芽率最高,但与20℃处理下的发芽率不存在显著差异;不同发芽床对发芽率的影响不存在显著性差异,但砂床可较好地抑制杂菌增长;无论是光照还是黑暗条件,黄芪种子均能正常发芽。在砂上15℃黑暗培养,黄芪种子发芽效果最好,其发芽率、发芽势和发芽指数分别为(98.5±0.65)%、(85.5±0.87)%和175.8±2.31,且发芽周期仅为4 d。结论:建议黄芪种子质量检验标准方法为将其在砂上于15℃黑暗培养,以发芽第2天、第4天分别作为初次、末次计数时间计算其发芽指标。该检验方法简单、可控,且发芽周期短,发芽率高。
OBJECTIVE： To select the optimum conditions for germination test of Astragalus membranaceus seed and provide reference for the formulation of testing rules ofA. membranaceus seed. METHODS： First, the dormancy ofA. membranaceus seeds were broken by soaking seed with 98% H2SO4 for 30 min and 35 ℃ warm water for 9 h, then treated with different temperatures of germination, different germinating beds and light conditions. Different treatment methods were evaluated by germination rate, germination potential and germination index. RESULTS： With the increase of temperature, germination rate, germination potential and germination index decreased. At 30 ℃, germination rate, germination index, and germination potential were significantly lower than those of seed with other treatments, in which the germination rate was the highest at 15 ℃; but there wasn＇t significant difference in germination rate of 15 ℃ and 20 ℃. There were no significant differences in germination rate of different germination beds, but sand bed could restrain bacterial growth well. Under both light and dark conditions, seed could normally germinate. The appropriate condition ofA. membranacus seed germination was at 15 ℃, sand bed and dark. The germination rate, germination potential and germination index were （98.5 ± 0.65） %, （85.5 ± 0.87） % and 175.8 ± 2.31, respectively. Meanwhile, the germination period was only 4 days. CONCLUSIONS： Suggested quality control method ofA. membranaceus seed is that at 15 ℃, sand bed and dark. The second day of germination as initial counting time and forth day as the last counting time are used to calculate germination index. This method is easy and controllable. It also has short germination period and high germination rate.