文献类型：期刊文献

英文题名：Sodium Alginate Launches MOF Defective Structures to Rapidly Construct Highly AChE-Like Nanozyme for Neuroprotection

作者：Chang, Xiang-Lei[1,2];Dan Zheng, Yi[1];Wang, Nan[1,3];Zhang, Xin-Ru[4];Qiang, Yin[4];Wang, Wei-Feng[1];Yang, Jun-Li[1]

第一作者：Chang, Xiang-Lei

通信作者：Wang, WF[1];Yang, JL[1];Qiang, Y[2]

机构：[1]Chinese Acad Sci, Lanzhou Inst Chem Phys, CAS Key Lab Chem Northwestern Plant Resources, Key Lab Nat Med Gansu Prov, Lanzhou 730000, Peoples R China;[2]Univ Chinese Acad Sci, Beijing 100049, Peoples R China;[3]Gansu Univ Chinese Med, Coll Pharm, Lanzhou 730000, Peoples R China;[4]Lanzhou Univ, Sch Pharm, Lanzhou 730000, Peoples R China

第一机构：Chinese Acad Sci, Lanzhou Inst Chem Phys, CAS Key Lab Chem Northwestern Plant Resources, Key Lab Nat Med Gansu Prov, Lanzhou 730000, Peoples R China

通信机构：[1]corresponding author), Chinese Acad Sci, Lanzhou Inst Chem Phys, CAS Key Lab Chem Northwestern Plant Resources, Key Lab Nat Med Gansu Prov, Lanzhou 730000, Peoples R China;[2]corresponding author), Lanzhou Univ, Sch Pharm, Lanzhou 730000, Peoples R China.

年份：2025

外文期刊名：SMALL METHODS

收录：;Scopus(收录号：2-s2.0-105015174418);WOS:【SCI-EXPANDED(收录号:WOS:001562321100001)】；

基金：This work was financially supported by the National Science and Technology Innovation 2030 Major Program (2023ZD0505300), National Key Research and Development Program (2024YFC3505603), CAS "Light of West China" Program, Major Program of the Lanzhou Institute of Chemical Physics, CAS (ZYFZFX-6), and the Excellent Doctoral Program of Gansu Province (24JRRA068).

语种：英文

外文关键词：acetylcholinesterase-like activity; metal-organic frameworks; nanozyme; neuroprotection

摘要：Organophosphorus (OP) nerve agents cause neurotoxicity through irreversible inhibition of acetylcholinesterase (AChE), while conventional therapeutic strategies are inadequate to fully restore cholinergic function. Artificial enzymes with AChE-like activity are urgently needed to provide sustainable catalytic capacity to address this limitation. Guided by the acid-base synergistic catalytic mechanism of the ester hydrolysis reaction, the 29-ZIF-8/SA composite with excellent AChE-like activity is successfully constructed by a defect engineering strategy with sodium alginate (SA) as a modulator. The mechanistic studies demonstrate that the basic site -OH groups introduced by SA, combined with the Lewis acid sites Zn2+ of ZIF-8, acted synergistically to form a bifunctional catalytic active site that enhances hydrolytic activity. Meanwhile, SA mediates the rapid formation of mesoporous architectures, improving substrate accessibility and mass transfer efficiency. In addition, 29-ZIF-8/SA demonstrates excellent environmental stability under harsh conditions and resists the inhibitory effect of methyl parathion (MP) on AChE activity. In vitro and in vivo experiments show that the 29-ZIF-8/SA composite exhibits cytoprotective properties to effectively attenuate MP-induced neurotoxicity. This work not only presents a designable method for the construction of highly active hydrolases, which are rarely reported, but also offers a new approach for nanozymes-based treatment of acute OP poisoning.