文献类型：期刊文献

英文题名：Simultaneous determination of oleanolic acid, ursolic acid, quercetin and apigenin in Swertia mussotii Franch by capillary zone electrophoresis with running buffer modifier

作者：Gao, Ruibin[1];Wang, Litao[2,3];Yang, Yan[4];Ni, Jingman[4];Zhao, Liang[2,3];Dong, Shuqing[2,3];Guo, Mei[1]

第一作者：Gao, Ruibin

通信作者：Dong, SQ[1]

机构：[1]Gansu Coll Tradit Chinese Med, Key Lab Chem & Qual Tradit Chinese Med, Coll Gansu Prov, Dept Pharm, Lanzhou 730000, Peoples R China;[2]Chinese Acad Sci, Lanzhou Inst Chem Phys, Key Lab Chem Northwestern Plant Resources, Lanzhou 730000, Peoples R China;[3]Chinese Acad Sci, Lanzhou Inst Chem Phys, Key Lab Nat Med Gansu Prov, Lanzhou 730000, Peoples R China;[4]Lanzhou Univ, Sch Pharm, Lanzhou 730000, Peoples R China

第一机构：甘肃中医药大学药学院（西北中藏药协同创新中心办公室）

通信机构：[1]corresponding author), Chinese Acad Sci, Lanzhou Inst Chem Phys, Key Lab Chem Northwestern Plant Resources, Lanzhou 730000, Peoples R China.

年份：2015

卷号：29

期号：3

起止页码：402

外文期刊名：BIOMEDICAL CHROMATOGRAPHY

收录：;Scopus(收录号：2-s2.0-84920873007);WOS:【SCI-EXPANDED(收录号:WOS:000347825800014)】；

基金：The authors acknowledge financial support from the National Nature Science Foundation of China (grant no. 20675085), the Science and Technology Support Xinjiang Project (XBXJ-2011-021), and Science and Technology Development Projects of Lanzhou (2010-1-156), and Gansu Province Chinese Medicine Talent Fund Project (GZK-2010-52).

语种：英文

外文关键词：Swertia mussotii Franch; pentacyclic triterpenoids; flavonoids; CZE; beta-cyclodextrin

摘要：The method of capillary zone electrophoresis (CZE) with direct UV detection was developed for the determination of oleanolic acid, ursolic acid, quercetin and apigenin. and then for the first time successfully applied to the analysis of four analytes in Swertia mussotii Franch and its preparations. Various factors affecting the CZE procedure were investigated and optimized, and the optimal conditions were: 50x10(-3)mol/L borate-phosphate buffer (pH9.5) with 5.0x10(-3)mol/L -cyclodextrin, 15kV separation voltage, 20 degrees C column temperature, 250nm detection wavelength and 5s electrokinetic injection time (voltage 20psi). Under the conditions, oleanolic acid, ursolic acid, quercetin and apigenin could be determined within the test ranges with a good correlation coefficient (r(2)>0.9991). The limits of detection for conditions, oleanolic acid, ursolic acid, quercetin and apigenin were 0.3415, 0.2003, 0.0062 and 0.2538 mu g/mL, respectively, and the intra- and inter-day relative standard deviations were no more than 4.72%. This procedure provided a convenient, sensitive and accurate method for simultaneous determination of oleanolic acid, ursolic acid, quercetin and apigenin in S. mussotii Franch. Copyright (c) 2014 John Wiley & Sons, Ltd.