文献类型：期刊文献

英文题名：EFFECT OF QUERCETIN ADMINISTRATION ON TRANSDIFFERENTIATION OF GLOMERULAR PODOCYTES TO MESENCHYMAL CELLS INDUCED BY TRANSFORMING GROWTH FACTOR

作者：Yang, Jing[1,2];Liu, Kai[1];Dai, Enlai[1]

第一作者：杨晶;杨静;Yang, Jing

通信作者：Dai, EL[1]

机构：[1]Gansu Univ Chinese Med, Lanzhou 730000, Peoples R China;[2]Lanzhou Univ, Hosp 2, Lanzhou 730000, Peoples R China

第一机构：甘肃中医药大学

通信机构：[1]corresponding author), Gansu Univ Chinese Med, Lanzhou 730000, Peoples R China.|[10735]甘肃中医药大学;

年份：2020

卷号：36

期号：4

起止页码：2605

外文期刊名：ACTA MEDICA MEDITERRANEA

收录：;Scopus(收录号：2-s2.0-85090153449);WOS:【SCI-EXPANDED(收录号:WOS:000552897900065)】；

基金：This work was supported by Scientific Research Project of Hubei Provincial Health and Family Planning Commission (WJ2015Z064).

语种：英文

外文关键词：Quercetin; TGF-beta 1; podocyte; mesenchymal cell transdifferentiation

摘要：Objective: The inhibitory effect of quercetin (Qu) on transforming growth factor - beta 1(TGF-beta 1)induced glomerular podocyte transdifferentiation (EMT) was analyzed. Methods: The in vitro primary cultured podocytes were divided into five groups using the random method. The control group was cultured in the normal mode. The model group was induced by TGF-beta 1 for 48 hours. Experimental group A was induced by quercetin for 24 hours, and subsequently induced by TGF-beta 1. At 24 hours, experimental group B was induced by quercetin and TGF-beta 1 for 48 hours. Experimental group C was induced by TGF-beta 1 for 24 hours, and subsequently treated with quercetin for 24 hours. The morphological changes of podocytes were observed under confocal laser microscopy. The expression of TGF-beta 1, ncphrin, WT-1, vimentin and alpha-SMA in podocytes were measured by reverse transcription polymerase chain reaction ( RT-PCR) and double staining flow cytometry. Results: Compared with the control group, the mRNA expression of TGF-beta 1 increased in the model group, when compared to the control group (P<0.05). Compared with the model group, the mRNA expression levels of TGF-beta 1 decreased in experimental groups A, B and C, when compared to the model group (P<0.05). Compared to the control group, the podocyte index growth slowed down, and the total cell apoptosis rate increased. However, when the podocyte index increased, the total cell apoptosis rate decreased in experimental groups A, B and C (P<0.05). Compared to the model group, the expression levels of podocyte marker proteins ncphrin and WT-1 increased, the expression levels of EMT marker proteins vimentin and alpha-SMA decreased (P<0.05), and the protein of experimental group A was tested. The expression is optimally improved. Conclusion: Quercetin can reduce the mRNA expression of TGF-beta 1 induced by TGF-beta 1 and the expression levels of EMT marker proteins alpha-SMA and vimentin, increase the expression of podocvte marker proteins ncphrin and WT-1, and inhibit podocyte orientation. Furthermore, mesenchymal cells are transdifferentiated.