文献类型：期刊文献

中文题名：基于b-FGF/Akt/Caspase-9探讨脱管散促进压力性损伤创面愈合机制

英文题名：To Explore the Machenlsm of Tuoguan Powder in Promoting PressureInjury Wound Healing Based on b-FGF/Akt/Caspase-9

作者：战青[1];张乐乐[2];杨淑珍[1];李巧梅[3];王波[4]

第一作者：战青

机构：[1]河西学院护理学院,甘肃张掖734000;[2]武警甘肃省总队医院,甘肃兰州730000;[3]甘肃中医药大学附属医院,甘肃兰州730000;[4]甘肃中医药大学,甘肃兰州730000

第一机构：河西学院护理学院,甘肃张掖734000

年份：2025

卷号：21

期号：9

起止页码：13

中文期刊名：亚太传统医药

外文期刊名：Asia-Pacific Traditional Medicine

基金：河西学院校长基金青年科研项目(QN2023016)。

语种：中文

中文关键词：压力性损伤;脱管散;碱性成纤维细胞生长因子;蛋白激酶B;半胱氨酸天冬氨酸酶-9

外文关键词：Pressure Injury;Tuoguan Powder;Basic Fibroblast Growth Factor;Protein Kinase B;Caspase-9

摘要：目的:探讨b-FGF、Akt和Caspase-9在压力性损伤创面组织不同时点的表达及相互作用。方法:实验分空白对照组(A)、模型组(B)、湿润烧伤膏组(C)、脱管散低(D)、中(E)、高剂量组(F),每组10只大鼠。利用局部组织缺血再灌注机制制备大鼠压力性损伤模型。分别于第3、7、14天,运用荧光定量PCR技术检测创面组织b-FGF mRNA表达,运用蛋白免疫印迹法(WB)技术检测创面组织b-FGF蛋白表达,运用ELISA技术测定创面组织p-Akt^(s473)含量,运用免疫组织化学法检测创面组织p-Caspase-9s196表达情况。结果:与A组相比,各组b-FGF mRNA、b-FGF的表达均高于A组,其中用药后第3天、7天的差异具有统计学意义(P<0.05);各组p-Akt^(s473)、p-Caspase-9s196表达均高于A组(P<0.05)。与B组相比,C、D、E、F组b-FGF mRNA、b-FGF的表达在用药后第3天、7天均高于B组(P<0.05),在用药后第14天表达下降;用药期间C、D、E、F组p-Akt^(s473)、p-Caspase-9s196的表达均高于B组(P<0.05)。与C组相比,D、E、F组b-FGF mRNA、b-FGF、p-Akt^(s473)、p-Caspase-9s196的表达均高于C组,用药后第3天差异具有统计学意义(P<0.05)。结论:脱管散可在压力性损伤创面愈合过程中调控b-FGF的表达,促进创面血管再生,避免瘢痕组织形成;通过调控p-Akt^(s473)、p-Caspase-9s196的表达,激活PI3K/Akt信号通路,抑制细胞凋亡,促进大鼠压力性损伤创面的愈合。
Objective:To explore the temporal expression and interactions of b-FGF,Akt,and Caspase-9 in pressure ulcer wound tissues.Methods:The rats were randomly divided into control group(A),model group(B),moist burn ointment group(C),Tuoguan powder low(D),medium(E),and high dose groups(F),with 10 rats in each group.The pressure injury model was established by local tissue ischemia-reperfusion mechanism in rats.On days 3,7,and 14 post-induction,the expression of b-FGF mRNA in wound tissue was detected by fluorescence quantitative PCR,the protein expression of b-FGF in wound tissue was detected by Western blot(WB)technique,the content of p-Akt ^(s473) in wound tissue was determined by ELISA,and the expression of p-Caspase-9 s196 in wound tissue was detected by immunohistochemistry.Results:Compared with group A,the expression of b-FGF mRNA,b-FGF in each group was higher than that in group A,and the difference was statistically significant on day 3 and 7(P<0.05).The expressions of p-AKt ^(s473) and p-Caspase-9S196 in each group were higher than those in group A(P<0.05).Compared with group B,the expression of b-FGF mRNA,b-FGF in groups C,D,E and F was significantly higher on the 3rd and 7th day after administration(P<0.05),and decreased on the 14th day after administration.The expressions of p-AKt ^(s473) and p-caspase-9 s196 in groups C,D,E and F were significantly higher than those in group B(P<0.05).Compared with group C,the expressions of b-FGF mRNA,b-FGF,p-AKt ^(s473) and p-caspase-9 s196 in groups D,E and F were higher than those in group C,and the differences were statistically significant on the third day after administration(P<0.05).Conclusion:Tuoguan Powder can regulate the expression of b-FGF in the process of pressure injury wound healing to promote wound angiogenesis and avoid scar tissue formation.It can promote the healing of pressure injury wounds in rats by regulating the expressions of p-Akt^(s473) and p-Caspase-9s196,activating PI3K/Akt signaling pathway and inhibiting cell apoptosis.