文献类型：期刊文献

中文题名：香椿子总黄酮体外抑菌作用研究

英文题名：In vitro antibacterial activity of total flavonoids from Fructus Toonae Sinensis

作者：王振恒[1,2];林丽[1,2];牛新迈[1];张星霞[1];晋玲[1,2]

第一作者：王振恒

机构：[1]甘肃中医学院;[2]中(藏)药资源研究所

第一机构：甘肃中医药大学

年份：2014

卷号：33

期号：2

起止页码：51

中文期刊名：中兽医医药杂志

外文期刊名：Journal of Traditional Chinese Veterinary Medicine

基金："十二五"国家科技支撑计划(2011BAI 05B02);甘肃中医学院教学改革项目(2012-QN03)

语种：中文

中文关键词：香椿子;总黄酮;体外抑菌作用

外文关键词：Fructus Toonae Sinensis;;total flavonoids;;antibacterial activity in vitro

摘要：目的:研究香椿子中总黄酮体外抑菌效果,与西药盐酸左氧氟沙星的体外抑菌作用进行对比,为今后临床用药提供实验依据。方法:对香椿子中总黄酮提取工艺优化,即95%乙醇提取,醇滤液浓缩,再经过正丁醇、乙醚进行萃取,浓缩得总黄酮。用药敏片法进行体外抑菌实验,明确抑菌效果。实验方法简便、易操作、重现性良好。结果:不同浓度香椿子总黄酮对大肠杆菌和金黄色葡萄球菌均有抑制作用;而不同浓度的盐酸左氧氟沙星对大肠杆菌有抑制作用,对金黄色葡萄球菌无抑制作用。
Objective: To investigate the antibacterial activity of total flavonoids from Fructus Toonae Sinensis in vitro by using Levofloxacin Hydroehloride as control. Methods: To optimize the extraction process of total flavonoids. Firstly, ethanol(95%) was used to extract and then the filtrate was concentrated. Afterwards, the concentrated filtrate was extracted with n-buty alcohol and aether. At last, total flavonoids were obtained. Drug sensitivity test was conducted and obtained the antibacterial range. The methods we used in this study were simple and showed good reproducibility. Results: Different concentrations of the total flavonoids from Fructus Toonae Sinensis could all inhibit the proliferation of E.coli and S.aureus, while under the same conditions,levofloxacine hydroehloride could only inhibit the proliferation of E.coli.