文献类型：期刊文献

中文题名：基于氧化应激及细胞凋亡探讨补气活血益精方对肾虚血瘀型少弱精子症大鼠的影响

英文题名：The effect of Qi-supplementing,blood-activating and essence-benefiting formula on rats with asthenospermia and oligospermia due to kidney deficiency and blood stasis based on oxidative stress and apoptosis

作者：常家庆[1];赵威威[1];马丽丽[2];鞠海超[1];杨佳树[1];王新平[3];邢喜平[3]

第一作者：常家庆

机构：[1]甘肃中医药大学,甘肃兰州730000;[2]兰州市第一人民医院,甘肃兰州730000;[3]甘肃中医药大学附属医院,甘肃兰州730000

第一机构：甘肃中医药大学

年份：2025

卷号：41

期号：16

起止页码：2324

中文期刊名：中国临床药理学杂志

外文期刊名：The Chinese Journal of Clinical Pharmacology

收录：;北大核心:【北大核心2023】；

基金：甘肃省自然科学基金资助项目(编号:25JRRA969);甘肃省卫生健康行业骨干人才基金资助项目(编号:甘卫人函[2023] 398号);第一批陇原青年英才培养基金资助项目(编号:甘人社通[2021] 404号);甘肃省中医药管理局基金资助项目(编号:GZKP-2023-15)。

语种：中文

中文关键词：补气活血益精方;肾虚血瘀;少弱精子症;氧化应激;细胞凋亡

外文关键词：Qi-supplementing;blood-activating and essence-enhancing formula;kidney deficiency and blood stasis;oligoasthenospermia;oxidative stress;apoptosis

摘要：目的 探究氧化应激及细胞凋亡在肾虚血瘀型少弱精子症(OAS)模型中的作用及补气活血益精方干预的机制。方法 用雷公藤多苷(GTW)灌胃的方法建立肾虚血瘀型OAS大鼠模型,将其随机分为模型组、左卡尼汀组、补气活血益精方低、中、高剂量实验组;另随机取8只大鼠为正常对照组。左卡尼汀组灌胃1.8 mL·kg^(-1)左卡尼汀口服液;补气活血益精方低、中、高剂量组分别灌胃予以7.87、15.75、31.50 g·kg^(-1)中药汤剂;空白组和模型组均灌胃等量0.9%NaCl, 6组大鼠每天定时给药1次,连续给药28 d。观察大鼠一般情况,测定大鼠睾丸及附睾指数;检测大鼠精子质量,苏木精-伊红染色法(HE)观察大鼠睾丸组织病理形态学变化,酶联免疫吸附测定(ELISA)检测睾丸组织活性氧(ROS)强度、过氧化氢酶(CAT)及超氧化物歧化酶(SOD)活力,实时荧光定量聚合酶链式反应(q-PCR)检测大鼠睾丸组织中Caspase-3、Bcl-2、Bax mRNA表达水平。结果 空白组、模型组、补气活血益精方低、中、高剂量组和左卡尼汀组睾丸指数分别为(0.83±0.09)%、(0.55±0.10)%、(0.55±0.07)%、(0.71±0.12)%、(0.81±0.08)%和(0.67±0.07)%,附睾指数分别为(0.36±0.05)%、(0.24±0.03)%、(0.25±0.04)%、(0.28±0.02)%、(0.35±0.06)%和(0.28±0.03)%,精子浓度分别为(24.11±11.64)、(4.65±2.48)、(6.75±3.81)、(11.60±7.78)、(21.72±7.81)和(23.22±8.80)×10^(6) sperm·mL^(-1),精子活力分别为(86.93±12.00)%、(33.46±16.13)%、(53.01±21.71)%、(63.15±24.35)%、(79.97±10.22)%和(75.83±25.05)%,ROS强度分别为597 926.11±87 518.20、925 239.02±95 539.79、846 676.84±64 867.76、784 277.73±81 354.32、658 228.04±82 768.68和725 740.12±87 846.36,CAT活力分别为(1.40±0.11)、(0.56±0.09)、(0.77±0.11)、(0.95±0.13)、(1.15±0.12)和(1.03±0.11)U·mg prot^(-1),SOD活力分别为(2.41±0.07)、(1.65±0.05)、(1.79±0.33)、(1.90±0.04)、(2.21±0.05)和(2.06±0.04)U·mg prot^(-1),Bcl-2 mRNA相对表达量分别为1.00±0.04、0.26±0.02、0.39±0.04、0.49±0.02、0.87±0.02、0.66±0.05,Bax mRNA相对表达量分别为1.00±0.05、1.78±0.07、1.50±0.04、1.39±0.02、1.12±0.04和1.27±0.04,Caspase-3 mRNA相对表达量分别为1.00±0.03、1.95±0.06、1.81±0.03、1.68±0.03、1.18±0.07和1.49±0.08。模型组的上述指标与空白组比较、高剂量组的上述指标与模型组比较、除附睾指数外左卡尼汀组的上述指标与模型组比较,在统计学上差异均有统计学意义(P<0.05,P<0.01)。结论 氧化应激及细胞凋亡在OAS大鼠精子质量及睾丸损伤中发挥多重调控作用,补气活血益精方可能通过抑制氧化应激及细胞凋亡,改善大鼠精子质量及睾丸功能。
Objective To explore the roles of oxidative stress and apoptosis in the renal deficiency and blood stasis type oligoasthenozoospermia(OAS)model,and to investigate the mechanism of the intervention by the Qi-supplementing,Blood-activating and Essence-nourishing formula.Methods The rat model of renal deficiency and blood stasis type oligoasthenozoospermia was established by intragastric administration of Gentiana macrophylla polysaccharides(GTW).The rats were randomly divided into the model group,the levocarnitine group,the low,medium and high doses of the Qi-supplementing,Blood-activating and Essence-nourishing formula groups;another 8 rats were randomly selected as the normal control group.The levocarnitine group was intragastrically administered 1.8 mL·kg^(-1) levocarnitine oral liquid;the low,medium and high doses of the Qi-supplementing,Blood-activating and Essence-nourishing formula groups were given 7.87,15.75 and 31.50 g·kg^(-1),respectively;the blank group and the model group were intragastrically administered the same amount of 0.9%NaCl.All 6 groups of rats were administered the drugs once daily and continuously for 28 days.The general conditions of the rats were observed;the testicular and epididymal indices were measured;the sperm quality was detected;the pathological morphology of the testicular tissue was observed by hematoxylin-eosin staining(HE);the activity of reactive oxygen species(ROS),catalase(CAT)and superoxide dismutase(SOD)in the testicular tissue was detected by enzyme-linked immunosorbent assay(ELISA);the mRNA expression levels of Caspase-3,Bcl-2 and Bax in the testicular tissue were detected by real-time fluorescence quantitative polymerase chain reaction(q-PCR).Results The testicular indices of the blank group,model group,low,medium and high doses of the Qi-supplementing,Blood-activating and Essence-nourishing formula group,and the levocarnitine group were(0.83±0.09)%,(0.55±0.10)%,(0.55±0.07)%,(0.71±0.12)%,(0.81±0.08)%,and(0.67±0.07)%,respectively;the epididymal indices were(0.36±0.05)%,(0.24±0.03)%,(0.25±0.04)%,(0.28±0.02)%,(0.35±0.06)%,and(0.28±0.03)%,respectively;the sperm concentrations were(24.11±11.64,4.65±2.48,6.75±3.81,11.60±7.78,21.72±7.81,23.22±8.80)×10^(6) sperm·mL^(-1),respectively;the sperm motility was(86.93±12.00)%,(33.46±16.13)%,(53.01±21.71)%,(63.15±24.35)%,(79.97±10.22)%,and(75.83±25.05)%,respectively;the ROS intensity was 597926.11±87518.20,925239.02±95539.79,846676.84±64867.76,784277.73±81354.32,658228.04±82768.68,and 725740.12±87846.36,respectively;the CAT activity was(1.40±0.11),(0.56±0.09),(0.77±0.11),(0.95±0.13),(1.15±0.12),and(1.03±0.11)U·mgprot^(-1),respectively;the SOD activity was(2.41±0.07),(1.65±0.05),(1.79±0.33),(1.90±0.04),and(2.21±0.05),and(2.06±0.04)U·mgprot^(-1),respectively.the relative expression levels of Bcl-2 mRNA were 1.00±0.04,0.26±0.02,0.39±0.04,0.49±0.02,0.87±0.02,and 0.66±0.05,respectively;the relative expression levels of Bax mRNA were 1.00±0.05,1.78±0.07,1.50±0.04,1.39±0.02,1.12±0.04,and 1.27±0.04,respectively;the relative expression levels of Caspase-3 mRNA were 1.00±0.03,1.95±0.06,1.81±0.03,1.68±0.03,1.18±0.07,and 1.49±0.08,respectively.The above-mentioned indicators of the model group compared with the blank group,the high-dose group compared with the model group,and the L-carnitine group except for the epididymal index compared with the model group,all showed statistically significant differences(P<0.05,P<0.01).Conclusion Oxidative stress and cell apoptosis play multiple regulatory roles in the sperm quality and testicular damage of OAS rats.The Qi-supplementing,activating blood,and tonifying essence formula may improve the sperm quality and testicular function of rats by inhibiting oxidative stress and cell apoptosis.