文献类型：期刊文献

中文题名：不同推拿手法对坐骨神经痛模型大鼠的镇痛作用及其镇痛机制研究

英文题名：Analgesic effect of different tuina manipulation on ischialgia model rats and its analgesic mechanism

作者：周强[1];冯喜莲[2];李姝睿[3];文新[2];刘乃嘉[2];王琼[4];赵峰[5];张静婧[6];肖玉霞[7]

第一作者：周强

机构：[1]甘肃中医药大学附属医院中医综合治疗区,甘肃兰州730020;[2]甘肃中医药大学附属医院针灸科,甘肃兰州730020;[3]甘肃中医药大学附属医院消毒供应中心,甘肃兰州730020;[4]甘肃中医药大学附属医院神经内科,甘肃兰州730020;[5]甘肃中医药大学附属医院神经外科,甘肃兰州730020;[6]甘肃中医药大学附属医院耳鼻咽喉头颈外科,甘肃兰州730020;[7]甘肃中医药大学附属医院畅家巷名中医门诊,甘肃兰州730000

第一机构：甘肃中医药大学第二附属医院

年份：2020

卷号：37

期号：6

起止页码：1

中文期刊名：甘肃中医药大学学报

外文期刊名：Journal of Gansu University of Chinese Medicine

基金：甘肃省高等学校科研项目(2014A-082);甘肃省自然科学基金项目(1508RJZA014)。

语种：中文

中文关键词：坐骨神经痛;推拿手法;镇痛作用;痛敏分数;β-内啡肽;5-羟色胺;P物质;大鼠;实验研究

外文关键词：ischialgia;tuina manipulation;analgesic effect;pain sensitivity score;β-endorphin(β-EP);5-hydroxytryptamine(5-HT);P-substance(SP);rats;experimental study

摘要：目的研究不同推拿手法对坐骨神经痛模型大鼠的镇痛作用及其对相关镇痛、致痛物质的影响。方法将50只雄性SD大鼠采用随机数字表法分为空白组、模型组、擦法治疗组、按法治疗组、拍法治疗组5组,每组10只。除空白组外,其余4组采用坐骨神经干结扎法复制坐骨神经痛大鼠模型,从术后第4天开始,各推拿治疗组大鼠于右后肢坐骨神经结扎处环跳穴及腓肠肌局部分别施以不同推拿手法(包括擦法、按法、拍法),每次20 min,每日1次,连续治疗5 d。5 d后检测各组大鼠的痛敏分数及血清5-羟色胺(5-HT)、β-内啡肽(β-EP)、P物质(SP)的含量。结果与空白组比较,模型组大鼠血清5-HT含量明显升高,痛敏分数及血清β-EP、SP含量明显降低,差异均有统计学意义(P<0.05);与模型组比较,各推拿治疗组大鼠血清5-HT含量明显降低,痛敏分数及血清β-EP、SP含量明显升高,差异均有统计学意义(P<0.05);各推拿治疗组之间比较差异均无统计学意义(P>0.05)。结论推拿手法可明显减轻坐骨神经痛模型大鼠的局部神经痛,具有显著的镇痛作用,其作用机制可能与升高大鼠血清镇痛物质的含量、减少致痛物质的释放、提高大鼠痛阈有关。
Objective To study the analgesic effect of different tuina manipulation on ischialgia model rats and the effects on related analgesic and algogenic substances.Methods Fifty male SD rats were divided into 5 groups including blank group,model group,scrubbing manipulation treatment group,pressing manipulation treatment group and patting manipulation treatment group,with 10 rats in each.Except the blank group,the remaining 4 groups replicated the model rats of sciatica with sciatic nerve trunk ligature.Starting from the 4 th day after the operation,the rats in each tuina treatment group were treated with different tuina manipulation(including rubbing,pressing and patting manipulation)respectively at the acupoint of Huantiao(GB 30)on the right hind limb sciatica nerve ligation spot and local gastrocnemius muscle,each time 20 minutes,once a day,continuous treatment for 5 days.After 5 days,the pain sensitivity score and the content of serum 5-hydroxytryptamine(5-HT),β-endorphin(β-EP)and P-substance(SP)of rats in each group were determined.Results Compared with the blank group,the content of serum 5-HT in rats of the model group significantly increased,the pain sensitivity score and the content of serumβ-EP and SP significantly decreased,and the difference was statistically significant(P<0.05).Compared with the model group,the content of serum 5-HT in rats of various tuina treatment groups significantly decreased,the pain sensitivity score and the content of serumβ-EP and SP significantly increased,and the difference was statistically significant(P<0.05).The difference was not statistically significant among various tuina treatment groups(P>0.05).Conclusion Tuina manipulation can markedly reduce the local neuralgia in ischialgia model rats,and has marked analgesic effect,whose action mechanism may be related to increasing the serum content of analgesic substances,reducing the release of algogenic substances,and increasing the pain threshold in rats.