文献类型：期刊文献

英文题名：Er Shen Wan extract reduces diarrhea and regulates AQP 4 and NHE 3 in a rat model of spleen-kidney Yang deficiency-induced diarrhea

作者：Xiong, Rui[1];Li, Wenbing[1,2];Li, Yidan[1];Zheng, Kaixuan[1];Zhang, Tingting[1];Gao, Mingyang[1];Li, Yun[1,3];Hu, Lin[1];Hu, Changjiang[1,2]

第一作者：Xiong, Rui

通信作者：Hu, CJ[1]

机构：[1]Chengdu Univ Tradit Chinese Med, Coll Pharm, Chengdu 610075, Sichuan, Peoples R China;[2]Sichuan Neogreen Pharmaceut Technol Dev Co Ltd, Chengdu 610081, Sichuan, Peoples R China;[3]Gansu Univ Tradit Chinese Med, Coll Pharm, Lanzhou 730000, Gansu, Peoples R China

第一机构：Chengdu Univ Tradit Chinese Med, Coll Pharm, Chengdu 610075, Sichuan, Peoples R China

通信机构：[1]corresponding author), Chengdu Univ Tradit Chinese Med, Coll Pharm, Chengdu 610081, Sichuan, Peoples R China.

年份：2018

卷号：98

起止页码：834

外文期刊名：BIOMEDICINE & PHARMACOTHERAPY

收录：;Scopus(收录号：2-s2.0-85039979283);WOS:【SCI-EXPANDED(收录号:WOS:000426104000104)】；

基金：This work was financially supported by grants from the General Program of the National Natural Science Foundation of China (81274085) and the Fund for Fostering Talents in Basic Science of the National Natural Science Foundation of China (J13100340), College of Pharmacy, Chengdu University of Traditional Chinese Medicine. We also acknowledge Prof. Peng Xi (College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan, PR China) and her research group for their technical support in this investigation. We also thank Sichuan Neo-Green Pharmaceutical Technology Development Co., Ltd for technical support and for providing instruments.

语种：英文

外文关键词：Er Shen Wan; Anti-diarrhea; AQP 4; NHE 3; VIP; NPY

摘要：Introduction: Er Shen Wan (ESW), a traditional Chinese medicinal formula comprised of Psoraleae Fructus (Babchi seeds, from Psoralea corylifolia Linn.) and Myristicae Semen (Nutmeg, from Myristica fragrans Houtt.), is widely used to treat spleen-kidney Yang deficiency (SKYD)-induced diarrhea. Previous studies have demonstrated preliminarily that the petroleum ether extract of ESW (ESWP) exhibits significant anti-diarrheal activity. The present study aimed to evaluate the anti-diarrhea activity of ESWP and to explore the underlying mechanisms with respect to fluid metabolism in a rat model of SKYD-induced diarrhea. Materials and methods: A high-performance liquid chromatography-diode array detector (HPLC-DAD) approach was developed and validated for qualitative and quantitative analyses of the main constituents of ESWP. SKYD model rats were established and treated with an effective dose (3.5 g/kg) of the extract for two weeks. Antidiarrheal activity and stool properties were observed. After the experiment, the appearance and histology of the intestines were evaluated. Serum levels of neuropeptide Y (NPY) and vasoactive intestinal peptide (VIP) were also determined. Furthermore, to characterize the regulation of aquaporin-4 (AQP 4) and Na+/H+ exchanger isoform 3 (NHE 3) in the colon, quantitative real-time RT-PCR (qRT-PCR), immunohistochemistry (IHC) and Western blotting (WB) were employed to detect mRNA and protein expression levels. Results: In the rat models, oral ESWP administration significantly reduced the diarrhea score and the number and weight of wet stools. Jejunal and ileac histological damage was impeded, and the histology score decreased. Serum VIP levels were significantly decreased, in contrast to NPY levels. In addition, AQP 4 and NHE 3 expression levels increased significantly. Conclusions: These results showed that ESWP's anti-diarrheal effect might at least partially involve the regulation of hormones intimately involved in maintaining fluid and electrolyte levels, as well as by increasing AQP 4 and NHE 3 expression levels and enhancing the absorption of Na+ and water.