文献类型：期刊文献

英文题名：Serum extracellular vesicle-derived miR-21-5p and miR-26a-5p as non-invasive diagnostic potential biomarkers for gastric cancer: A preliminary study

作者：Wang, Jun-Hong[1,2];Bai, Zhao-zhao[3];Niu, Xing-dong[4];Zhu, Cheng-lou[1];Liang, Tong[1];Hu, Yong-li[1];Gao, Zhen-Hua[2];Da, Ming-Xu[1,5]

第一作者：王谨慧;Wang, Jun-Hong

通信作者：Da, MX[1];Gao, ZH[2]

机构：[1]Lanzhou Univ, Clin Med Coll 1, 222 Tianshui South Rd, Lanzhou 730000, Peoples R China;[2]Gansu Univ Tradit Chinese Med, Peoples Hosp Baiyin 1, Affiliated Hosp 3, Dept Gen Surg, Baiyin 730900, Peoples R China;[3]Ningxia Med Univ, Sch Clin Med, Yinchuan, Peoples R China;[4]Gansu Univ Chinese Med, Gansu Prov Hosp, Clin Med Coll 1, Lanzhou, Peoples R China;[5]Gansu Prov Hosp, Dept Surg Oncol, Lanzhou, Peoples R China

第一机构：Lanzhou Univ, Clin Med Coll 1, 222 Tianshui South Rd, Lanzhou 730000, Peoples R China

通信机构：[1]corresponding author), Lanzhou Univ, Clin Med Coll 1, 222 Tianshui South Rd, Lanzhou 730000, Peoples R China;[2]corresponding author), Gansu Univ Tradit Chinese Med, Peoples Hosp Baiyin 1, Affiliated Hosp 3, Dept Gen Surg, Baiyin 730900, Peoples R China.|[10735]甘肃中医药大学;

年份：2024

外文期刊名：INTERNATIONAL JOURNAL OF BIOLOGICAL MARKERS

收录：;Scopus(收录号：2-s2.0-85196289743);WOS:【SCI-EXPANDED(收录号:WOS:001249111200001)】；

基金：The author(s) disclosed receipt of the following financial supportfor the research, authorship, and/or publication of this article: This study was supported by grants from the Science and Technology Planning Project of Gansu Province (Grant No. 21CX6FD163), the Second Group of Longyuan Young Talents in Gansu Province (Grant No. 2023-11) and the Science and Technology Planning Project of Baiyin City (Grant No.2021-1-18Y).

语种：英文

外文关键词：Extracellular vesicles; serum; miRNAs; diagnosis; gastric cancer

摘要：Purpose: Gastric cancer is the most common malignancy worldwide and is the third leading cause of cancer-related deaths, urgently requiring an early and non-invasive diagnosis. Circulating extracellular vesicles may emerge as promising biomarkers for the rapid diagnosis in a non-invasive manner. Methods: Using high-throughput small RNA sequencing, we profiled the small RNA population of serum-derived extracellular vesicles from healthy controls and gastric cancer patients. Differentially expressed microRNAs (miRNAs) were randomly selected and validated by reverse transcription-quantitative real-time polymerase chain reaction. Receiver operating characteristic curves were employed to assess the predictive value of miRNAs for gastric cancer. Results: In this study, 193 differentially expressed miRNAs were identified, of which 152 were upregulated and 41 were significantly downregulated. Among the differently expressed miRNA, the expression levels of miR-21-5p, miR-26a-5p, and miR-27a-3p were significantly elevated in serum-derived extracellular vesicles of gastric cancer patients. The miR-21-5p and miR-27a-3p were closely correlated with the tumor size. Moreover, the expression levels of serum miR-21-5p and miR-26a-5p were significantly decreased in gastric cancer patients after surgery. Conclusions: The present study discovered the potential of serum miR-21-5p and miR-26a-5p as promising candidates for the diagnostic and prognostic markers of gastric cancer.