文献类型：期刊文献

英文题名：Transcriptomic and Metabolomic Analyses Provide Insights Into the Flavonoid Biosynthesis in Dangshen

作者：Liu, Xuxia[1];Ma, Haitang[1];Liu, Xiaoling[1];Wang, Xin[1];Chen, Zhengjun[1];Yang, Jie[2];Luo, Wenrong[3];Li, Qin[4];Yang, Fude[1];Li, Fang[1]

第一作者：刘晓霞;刘旭旭

通信作者：Yang, FD[1];Li, F[1]

机构：[1]Gansu Univ Chinese Med, Sch Pharm, Lanzhou, Peoples R China;[2]Beijing Univ Chinese Med, Sch Tradit Chinese Med, Beijing, Peoples R China;[3]Gansu Prov Hosp Chinese Med, Inst Pharm, Lanzhou, Peoples R China;[4]Gansu Univ Chinese Med, Sch Basic Med, Lanzhou, Peoples R China

第一机构：甘肃中医药大学

通信机构：[1]corresponding author), Gansu Univ Chinese Med, Sch Pharm, Lanzhou, Peoples R China.|[10735]甘肃中医药大学;

年份：2025

外文期刊名：PHYTOCHEMICAL ANALYSIS

收录：;Scopus(收录号：2-s2.0-85214026670);WOS:【SCI-EXPANDED(收录号:WOS:001389043700001)】；

基金：We thank Gansu University of Chinese Medicine and BIOTREE Co. Ltd. for providing an experimental platform and research assistance. This research was funded by the National Natural Science Foundation of China (Grant Number 82160714), the Science and Technology Program of Gansu Province (Grant Numbers 21JR1RA271, 22JR5RA590, 23JRRA1369), the Key Technologies Research and Development Program (Grant Number 2018YFC1706305), the University Project of Gansu Province (Grant Number 2021CYZC-40), the Gansu Education Department Program (Grant Number 2023CXZX-740), the Key Research and Development Program of Gansu Province (Grant Number 23YFFA0069), the Gansu Province Educational Science and Technology Innovation Project (Grant Numbers 2022A-064, 2023A-297), the Lanzhou Municipal Bureau of Science and Technology School-Enterprise Co-operation Project (Grant Number 2023-RC-9), and the Open Project of Gansu Traditional Chinese Medicine Research Center for 2022-2023 (Grant Number zyzx-2023-10).

语种：英文

外文关键词：Dangshen; flavonoid; metabolomics; transcriptome sequencing

摘要：IntroductionDangshen (DS) has been used for hundreds of years as a traditional Chinese medicine. It has a wide range of biological activities. Flavonoids are one of the important bioactive components with strong free radical scavenging and antioxidant capacity in DS. However, the biosynthesis process of flavonoids in DS remains unclear.ObjectiveThe aim of this study was to understand the biosynthesis molecular mechanism of flavonoids in DS.MethodsIn this study, metabolomics research and transcriptome sequencing for DS were carried out. Transcript and metabolite profiles were generated by high-throughput RNA sequencing (RNA-seq) data analysis and liquid chromatography-tandem mass spectrometry, respectively.ResultsIn total, 256 metabolites were identified in the root, stem, leaf, and flower of DS using untargeted metabolomics. Among them, 55 flavonoids, including pinobanksin, butein, fustin, pelargonidin, apigenin, luteolin, and eriodictyol, were closely related to flavonoid metabolism, and most of them were upregulated in different tissues of DS. Furthermore, the differentially expressed genes identified by transcriptomics were mainly enriched in the biosynthesis of flavonoid, isoflavonoid, flavone, and flavonol. A number of genes, including ANS, CCOAOMT, CHI, CHS, CYP75B1, CYP75A, CYP93B2_16, CYP98A/C3'H, DFR, F3H, FLS, and HCT, may regulate the production of flavonoids in different tissues of DS. An integrated analysis of transcriptome and metabolome revealed the flavonoid biosynthetic network in DS and elucidated the diversity of flavonoid biosynthetic pathway in roots, stems, leaves, and flowers of DS.ConclusionOur findings provide a molecular basis and new insights into flavonoid biosynthesis in DS and lay the foundation for breeding new valuable DS cultivars.