文献类型：期刊文献

中文题名：玫瑰花醇提部位荧光特性研究

英文题名：Study on fluorescence characteristics of ethanol extract from rosae rugosae flos

作者：蒋朝辉[1,2];石振萍[1];邵士俊[2];杨扶德[1];冯金梁[3]

第一作者：蒋朝辉

机构：[1]甘肃中医药大学药学院,兰州730000;[2]中国科学院兰州化学物理研究所,兰州730000;[3]甘肃康乐药业有限责任公司,兰州730300

第一机构：甘肃中医药大学药学院（西北中藏药协同创新中心办公室）

年份：2021

卷号：12

期号：4

起止页码：1475

中文期刊名：食品安全质量检测学报

外文期刊名：Journal of Food Safety and Quality

收录：CSTPCD;;北大核心:【北大核心2020】；

基金：甘肃省科技小巨人企业培育计划项目(17CXIJA084);2018年度甘肃省委组织部人才发展专项资金项目[甘组(2018)23号];2018年兰州市人才创新创业科技计划项目(2017-RC-112);中科院兰州化物所“十三五”135重大突破项目(2016-2020)。

语种：中文

中文关键词：玫瑰花;三维荧光光谱;荧光特性;质量评价

外文关键词：rosae rugosae flos;three-dimensional fluorescence spectrum;fluorescence characteristics;quality evaluation

摘要：目的建立荧光光谱法测定玫瑰花醇提部位荧光特性的研究方法。方法采用荧光分析法结合紫外光谱测定玫瑰花醇提部位的光谱特征,并对浓度、温度以及放置时间等因素对荧光特性的影响进行研究。结果玫瑰醇提部位在Ex 287 nm/Em 364 nm存在一荧光特征峰,其荧光强度受浓度影响较大,在0.1~0.4 mg/mL浓度范围内,荧光强度随浓度的增大而增强;玫瑰花醇提部位荧光强度随温度的升高而降低;此外,在48 h内,其荧光强度较为稳定。结论玫瑰花醇提部位存在明显的荧光特征峰,且甘肃、山东及贵州3个不同产地荧光特征峰在强度上存在一定差异,为进一步完善玫瑰花药材质量评价系统提供了理论基础。。
Objective To establish a method for determining the fluorescence characteristics of rosae rugosae flos ethanol extract by fluorescence spectrometry.Methods The spectral characteristics of rosae rugosae flos ethanol extract were determined by fluorescence analysis combined with ultraviolet spectroscopy,and the effects of concentration,temperature and placing time on the fluorescence characteristics were studied.Results There was a characteristic fluorescence peak at Ex 287 nm/Em 364 nm at the ethanol extract site of rosae rugosae flos.The fluorescence intensity was greatly affected by the concentration.In the concentration range of 0.1–0.4 mg/mL,the fluorescence intensity was enhanced with the increase of concentration.The fluorescence intensity of rosae rugosae flos ethanol extract decreased with the increase of temperature.In addition,the fluorescence intensity was relatively stable within 48 h.Conclusion There are significant fluorescence characteristic peaks in the ethanol extract part of rosae rugosae flos,and there are some differences in fluorescence characteristic peaks from 3 different origins,Gansu,Shandong and Guizhou.It provides a theoretical basis for further improving the quality evaluation system of rose.