文献类型：期刊文献

英文题名：Network pharmacology-based identification of major component of Angelica sinensis and its action mechanism for the treatment of acute myocardial infarction

作者：Niu, Xiaowei[1,2];Zhang, Jingjing[2,3];Ni, Jinrong[4];Wang, Runqing[1,2];Zhang, Weigiang[5];Sun, Shaobo[5];Peng, Yu[2,6];Bai, Ming[2,6];Zhang, Zheng[2,6]

第一作者：Niu, Xiaowei

通信作者：Zhang, Z[1];Zhang, Z[2]

机构：[1]Lanzhou Univ, Sch Clin Med 1, Lanzhou, Gansu, Peoples R China;[2]Lanzhou Univ, Hosp 1, Gansu Key Lab Cardiovasc Dis, Lanzhou, Gansu, Peoples R China;[3]Baiyin Second Peoples Hosp, Dept Internal Med, Baiyin, Gansu, Peoples R China;[4]Lanzhou Univ, Hosp 1, Dept Cardiovasc Surg, Lanzhou, Gansu, Peoples R China;[5]Gansu Univ Chinese Med, Coll Integrated Tradit Chinese & Western Med, Lanzhou, Gansu, Peoples R China;[6]Lanzhou Univ, Hosp 1, Dept Cardiol, Lanzhou, Gansu, Peoples R China

第一机构：Lanzhou Univ, Sch Clin Med 1, Lanzhou, Gansu, Peoples R China

通信机构：[1]corresponding author), Lanzhou Univ, Hosp 1, Gansu Key Lab Cardiovasc Dis, Lanzhou, Gansu, Peoples R China;[2]corresponding author), Lanzhou Univ, Hosp 1, Dept Cardiol, Lanzhou, Gansu, Peoples R China.

年份：2018

卷号：38

期号：6

外文期刊名：BIOSCIENCE REPORTS

收录：;Scopus(收录号：2-s2.0-85056221981);WOS:【SCI-EXPANDED(收录号:WOS:000449352700002)】；

基金：This work was supported by the Research Project of Gansu Provincial Administration of Traditional Chinese Medicine [grant number GZK-2018-48].

语种：英文

摘要：Background: To decipher the mechanisms of Angelica sinensis for the treatment of acute myocardial infarction (AMI) using network pharmacology analysis. Methods: Databases were searched for the information on constituents, targets, and diseases. Cytoscape software was used to construct the constituent-target-disease network and screen the major targets, which were annotated with the DAVID (Database for Annotation, Visualization and Integrated Discovery) tool. The cardioprotective effects of Angelica sinensis polysaccharide (ASP), a major component of A. sinensis, were validated both in H9c2 cells subjected to simulated ischemia by oxygen and glucose deprivation and in rats with AMI by ligation of the left anterior coronary artery. Results: We identified 228 major targets against AMI injury for A. sinensis, which regulated multiple pathways and hit multiple targets involved in several biological processes. ASP significantly decreased endoplasmic reticulum (ER) stress-induced cell death both in vitro and in vivo. In ischemia injury rats, ASP treatment reduced infarct size and preserved heart function. ASP enhanced activating transcription factor 6 (ATF6) activity, which improved ER-protein folding capacity. ASP activated the expression of p-AMP-activated protein kinase (p-AMPK) and peroxisome proliferator-activated receptor gamma coactivator 1 alpha (PGC1 alpha). Additionally, ASP attenuated levels of proinflammatory cytokines and maintained a balance in the oxidant/antioxidant levels after AMI. Conclusion: In silico analysis revealed the associations between A. sinensis and AMI through multiple targets and several key signaling pathways. Experimental data indicate that ASP protects the heart against ischemic injury by activating ATF6 to ameliorate the detrimental ER stress. ASP's effects could be mediated via the activation of AMPK-PGC1 alpha pathway.