文献类型：期刊文献

英文题名：Antitumor effect of N-succinyl-chitosan nanoparticles on K562 cells

作者：Luo, Huiying[1];Li, Juan[2];Chen, Xuan[2]

第一作者：罗慧英

通信作者：Luo, HY[1]

机构：[1]Gansu Coll Tradit Chinese Med, Dept Pharmacol, Lanzhou 730000, Peoples R China;[2]Lanzhou Univ, Ctr Mol Biol, Hosp 1, Lanzhou 730000, Peoples R China

第一机构：甘肃中医药大学

通信机构：[1]corresponding author), Gansu Coll Tradit Chinese Med, Dept Pharmacol, 35 Dingxi E Rd, Lanzhou 730000, Peoples R China.|[10735]甘肃中医药大学;

年份：2010

卷号：64

期号：8

起止页码：521

外文期刊名：BIOMEDICINE & PHARMACOTHERAPY

收录：;Scopus(收录号：2-s2.0-77956882724);WOS:【SCI-EXPANDED(收录号:WOS:000283571100003)】；

语种：英文

外文关键词：N-succinyl-chitosan nanoparticles; Apoptosis; Necrosis

摘要：Polymer nanoparticles have become attractive for its prominent property recent years. In this paper, antitumor effects of N-succinyl-chitosan nanoparticles (NSCNP), a nanoparticles derivated from chitosan, were evaluated in K562 cells, including cell viability comparison, cell morphology analysis, DNA fragmentation detection, cell surface potential and mitochondrial membrane potential (MMP) measurement, intracellular ROS and Ca2+ concentration evaluation. Our results revealed NSCNP could inhibit the proliferation of K562 with an IC50 of 14.26 mu g/ml (24 h); decrease the zeta potential; disrupt the mitochondrial membrane potential; increase ROS generation and Ca2+ concentration. Cytomorphology study and DNA fragment analysis reveal characteristics of apoptosis and necrosis, indicating that the antitumor effect of NSCNP achieved by necrosis and apoptosis induction in K562 cells. (C) 2009 Elsevier Masson SAS. All rights reserved.