文献类型：期刊文献

中文题名：羌活种子发育过程中形态及生理生化指标动态变化特征

英文题名：Changes of morphological,physiological,and biochemical indices during seed development of Notopterygium incisum Ting ex H.T.Chang

作者：耿蓓蕊[1];高洁[1];辜晓英[1];晋玲[1,2,3];陈红刚[1,3];赵文龙[1,2,3]

第一作者：耿蓓蕊

机构：[1]甘肃中医药大学药学院,兰州730101;[2]陇药产业创新研究院,兰州730101;[3]甘肃省珍稀中药资源评价与保护利用工程研究中心,兰州730101

第一机构：甘肃中医药大学药学院（西北中藏药协同创新中心办公室）

年份：2024

卷号：44

期号：10

起止页码：1539

中文期刊名：西北植物学报

外文期刊名：Acta Botanica Boreali-Occidentalia Sinica

收录：CSTPCD;;北大核心:【北大核心2023】；CSCD:【CSCD2023_2024】；

基金：甘肃省自然科学基金项目(23JRRA1208);甘肃省科技重大专项(23ZDFA013-1);甘肃省高等学校青年博士基金项目(2022QB-093);西北中藏药省部共建协同创新中心开放基金项目(Xbzzy-2022-03);中药保障与创新能力提升项目(甘中医药综函[2024]14号);中央引导地方科技发展资金项目(24ZYQA041)。

语种：中文

中文关键词：羌活;种子发育;外观形态;生理生化指标

外文关键词：Notopterygium incisum Ting ex H.T.Chang;seed development;physiological and biochem-ical indicators;appearance and morphology

摘要：【目的】探讨羌活种子发育过程中形态及生理生化指标变化规律,为羌活引种驯化、优质种质资源培育提供理论依据。【方法】采用定期观测采样及冷冻切片法,对不同发育阶段的羌活种子形态及生理生化指标进行测定。【结果】羌活种子发育过程中,种子大小、果翅厚度先升后降,果翅宽度升高,外种皮颜色由明亮的蓝绿色转为黄褐色;贮藏物质淀粉、可溶性糖、可溶性蛋白含量升高;淀粉酶活性小范围波动,琥珀酸脱氢酶活性升高,葡萄糖-6-磷酸脱氢酶活性交替升降,丙酮酸激酶活性呈“M”形变化,POD活性降低,而SOD活性先降后升;GA3、IAA和ZR含量降低,ABA含量升高;种子形态指标与SOD活性极显著负相关,贮藏物质含量与种子厚度极显著负相关,而与果翅宽度极显著正相关。【结论】在羌活种胚发育至心形胚前期过程中,外种皮由蓝绿色转为黄褐色,贮藏物质含量升高,关键酶活性波动变化,促生长激素含量波动降低,而胁迫激素含量升高。
[Objective]The study aims to explore the changes of morphological,physiological,and biochemical indexes during the development of Notopterygium incisum seeds,and to provide theoretical basis for the introduction and domestication of N.incisum and the cultivation of high-quality germplasm resources.[Methods]The morphological,physiological,and biochemical indexes of N.incisum seeds at different developmental stages were determined by observation samplings and the frozen section method.[Results]During the development of N.incisum seeds,seed size and wing thickness were first increased and then decreased,while wing width was increased.The color of the outer seed coat was changed from bright blue-green to yellow-brown.The contents of starch,soluble sugar,and soluble protein were in-creased.Amylase activity was fluctuated in a small range;succinate dehydrogenase activity was increased;glucose-6-phosphate dehydrogenase activity was increased and decreased alternately;pyruvate kinase activ-ity showed“M”-shape changes.POD activity was decreased while SOD activity was decreased.GA3,IAA,and ZR contents were decreased,while ABA contents were increased.The morphological indexes of the seeds were negatively correlated with the activity of SOD.The content of the storage materials was negatively correlated with the seed thickness,but positively correlated with the width of the fruit wings.[Conclusion]When N.incisum embryo is developed to pre-cardioid embryo,the outer seed coat is changed from blue-green to yellow-brown,the content of storage material is increased,and the activity of key en-zymes is fluctuated and decreased.The content of growth-promoting hormones is fluctuated and de-creased,while the content of stress hormones is increased.