文献类型：期刊文献

中文题名：当归补血汤通过PI3K/Akt通路对AngⅡ诱导肥大心肌细胞的保护作用

英文题名：Protective Effect of Danggui Buxuetang on Hypertrophic Cardiomyocytes Induced by Angiotensin Ⅱ in Patients with Hypertrophy by PI3K/Akt Pathway

作者：徐厚谦[1];颜春鲁[1];张永花[1];金华[1];何建新[1]

第一作者：徐厚谦

机构：[1]甘肃中医药大学中医临床学院

第一机构：甘肃中医药大学中医临床学院

年份：2018

卷号：24

期号：2

起止页码：135

中文期刊名：中国实验方剂学杂志

外文期刊名：Chinese Journal of Experimental Traditional Medical Formulae

收录：CSTPCD;;北大核心:【北大核心2017】；CSCD:【CSCD_E2017_2018】；

基金：甘肃省自然科学基金项目(145RJZA170)

语种：中文

中文关键词：当归补血汤;血管紧张素II;LY294002阻断剂;心肌细胞肥大;磷脂酰肌醇3激酶(P13K)/丝氨酸苏氨;酸激酶(Akt)信号通路

外文关键词：Danggui Buxuetang; angiotensin Ⅱ ; LY294002 blocker; ardiomyocyte hypertrophy;phosphatidylinositol 3-kinase （PI3K） /serine threonine kinase （Akt） signaling pathway

摘要：目的：研究当归补血汤对肥大心肌细胞的保护作用及其与磷脂酰肌醇3激酶（P13K）／丝氨酸苏氨酸激酶（Akt）／内皮型一氧化氮合酶（eNOS）／-氧化氮（NO）信号转导通路的关系。方法：体外培养H9C2心肌细胞，采用a．肌动蛋白（a-actin）抗体进行免疫组化法鉴定心肌细胞；用血管紧张素Ⅱ（AngII）诱导H9C2心肌细胞肥大模型，通过BCA蛋白测定法测定心肌细胞总蛋白含量，比较空白组和模型组蛋白含量的不同，以验证AngⅡ诱导H9C2心肌细胞肥大的模型；取同代生长状态良好的细胞分为空白组，模型组，LY294002阻断剂组和10％含药血清组，蛋白免疫印迹法（Westernblot）检测各组心肌细胞P-Akt，Akt，P-eNOS，eNOS等信号通路的相关蛋白表达，硝酸还原酶法检测各组细胞培养液中NO浓度。结果：免疫组化法鉴定细胞，棕黄色细密颗粒位于细胞浆，其鉴定结果符合心肌细胞特征；BCA测定细胞蛋白，模型组较空白组蛋白表达增加（P〈0．05），AngII诱导H9C2心肌细胞肥大的模型建立；Westernblot结果显示模型组较空自组p-Akt，Akt，eNOS蛋白表达减少（P〈0．05）；含药血清组较模型组p-Akt，Akt，eNOS蛋白表达增加（P〈0．05），LY294002阻断剂组可消减含药血清的作用。结论f当归补血汤含药血清对AngⅡ诱导心肌细胞肥大起保护作用，其机制之一可能是通过调控心肌细胞P13K／Akt信号通路实现的。
Objective： To study the protective effect of Danggui Buxuetang on hypertrophic cardiomyocytes and its relationship with phosphatidylinositol 3-kinase （PI3K） /serine threonine kinase （Akt） / endothelial nitric oxide synthase （eNOS） -nitric oxide （NO） signal transduction pathway. Method： H9C2 cardiomyoeytes were cultured in vitro, α-actin antibody was used to identify cardiomyocytes by immunohistochemistry. Angiotensin Ⅱ （Ang Ⅱ ） was used to induce the H9C2 cardiomyocyte hypertrophy model. The total protein content of cardiomyocytes was measured by BCA protein assay. The protein contents of blank group and model group were compared to verify the model of Angiotensin-induced H9C2 cardiomyocyte hypertrophy. The cells were divided into blank group, cell model group, LY294002 blockade group and 10% serum group. Western blot was used to detect relevant protein expressions of p-Akt, Akt, p-eNOS, eNOS and other signaling pathways. Nitric acid reductase assay was used to detect the NO concentration in each cell culture medium. Result： The immunohistochemical method was used to identify that cells and brownish yellow fine particles were located in the cytoplasm. The results were consistent with the characteristics of cardiomyocytes. BCA was used to determine cell protein. Compared with blank group, the content of the histone group was increased （P 〈 0. 05）. Western blot analysis showed that p-Akt, Akt and eNOS protein expressions in model group were lower than those in blank group （P 〈 0.05） , p-Akt, Akt and eNOS protein expressions in the serum-containing group were higher than those in model group （P 〈 0.05 ）, LY294002 blockade group removed the effect of the drug- containing serum. Conclusion： Danggui Buxuetang-containing serum has a protective effect on Ang I1-induced cardiomyocyte hypertrophy. One of its mechanisms may be the regulation of cardiomyocyte PI3K /Akt signaling pathway.