文献类型：期刊文献

中文题名：多环芳烃诱发小鼠实体瘤及其对白介素-2及白介素-6的影响

英文题名：Solid Tumors Induced by Polycyclic Aromatic Hydrocarbons in Mice and Relevant Effects on Interleukin-2 and Interleukin-6

作者：王秋兰[1];万学中[1]

第一作者：王秋兰

机构：[1]甘肃中医药大学临床医学院

第一机构：甘肃中医药大学临床医学院

年份：2015

卷号：32

期号：9

起止页码：880

中文期刊名：环境与职业医学

外文期刊名：Journal of Environmental & Occupational Medicine

收录：CSTPCD;;北大核心:【北大核心2014】；CSCD:【CSCD_E2015_2016】；

基金：甘肃省自然科学基金项目(编号:145RJZA235);甘肃省高等学校基本科研业务费项目(编号:2012-5)

语种：中文

中文关键词：多环芳烃;蒽;苯并[a]芘;苯并[g;h;i]苝;小鼠;实体瘤;白介素-2;白介素-6

外文关键词：polycyclic aromatic hydrocarbon; anthracene; benzo[a]pyrene; benzo[g, h,i]perylene; mouse; solidtumor; interleukin-2; interleukin-6

摘要：[目的]探索多环芳烃中单一标准品蒽、苯并[a]芘(Ba P)、苯并[g,h,i]苝诱发小鼠实体瘤的情况;通过白介素-2(IL-2)、白介素-6(IL-6)的测定,探索其对机体免疫功能的影响。[方法]无特定病原体(SPF)级昆明种小鼠120只,雌雄各半,体重(20±2)g。取20只预实验确定染毒剂量,其余100只随机分为5组,每组20只,分别为二甲基亚砜对照组、蒽50 mg/kg组、Ba P 10mg/kg组、Ba P 20mg/kg组,苯并[g,h,i]苝5 mg/kg组,腹腔注射,每日一次,连续10次。3个月后采血,酶联免疫吸附试验(ELISA)测定血清IL-2及IL-6。取肝、肾、胃、肺,进行病理组织学检查。[结果]苯并[g,h,i]苝组5 mg/kg组、Ba P 10 mg/kg组、Ba P 20 mg/kg、蒽50 mg/kg组小鼠肝癌、胃癌、肾癌发生率分别依次为21.1%(4/19)、26.3%(5/19)、35.3%(6/17)、27.8%(5/18),21.1%(4/19)、0.0%(0/19)、41.2%(7/17)、0.0%(0/18),0.0%(0/19)、0.0%(0/19)、11.8%(2/17)、0.0%(0/18);肝癌、胃癌、肾癌癌前病变率依次为68.4%(13/19)、73.7%(14/19)、64.7%(11/17)、55.6%(10/18),78.9%(15/19)、68.4%(13/19)、29.4%(5/17)、27.8%(5/18),42.1%(8/19)、47.4%(9/19)、58.8%(10/17)、33.3%(6/18)。各实验组小鼠癌前病变率均高于对照组(P<0.05);Ba P 20 mg/kg组胃癌发生率及癌前病变率高于10 mg/kg组(P<0.05);实验组肺脏未见明显损害。各实验组小鼠血清IL-2、IL-6水平均低于对照组(P<0.01)。[结论]蒽、Ba P和苯并[g,h,i]苝腹腔注射后对肝脏、胃和肾脏均有明显的损害,肺脏未出现病变,提示多环芳烃的靶器官不同。实验各组IL-2、IL-6浓度均明显低于对照组,提示多环芳烃对机体的免疫功能有明显的抑制作用。推测苯并[g,h,i]苝致癌性最强,其次为Ba P,蒽致癌性最低。
[ Objective ] To observe solid tumors in mice induced by single standards of polycyclic aromatic hydrocarbons including anthracene, benzo[a]pyrene, benzo[g, h, i]perylene, and to explore relevant effects on immune function by measuring serum interleukin-2 （IL-2） and interleukin-6 （IL-6）. [ Methods ] The study used a total of 120 specific pathogen free （SPF） Kunming mice, half male and half female, with body weights at （20 ＋ 2） g. Twenty mice were used for pilot experiment to determine dosage. Then the remaining 100 mice were randomly divided into dimethyl sulfoxide control group, anthracene 50mg/kg group, benzo[a]pyrene 10 mg/kg group, benzo[a]pyrene 20 mg/kg group, and benzo[g, h, i]perylene 5 mg/kg group, with 20 mice in each group. The five groups were injected with assigned chemicals intraperitoneally daily for 10 days. Blood sample was collected after three months and serum IL-2 ＆ IL-6 were detected by enzyme linked immunosorbent assay （ELISA）. Liver, kidney, stomach, and lung of mice were subjected to histopathological examination. [ Results ] Of the mice in the benzo[g, h, i]perylene 5 mg/kg group, benzo[a]pyrene 10mg/kg group, benzo[a]pyrene 20mg/kg group, and anthracene 50mg/kg group, the cancer incidences in liver were 21.1% （4/19）, 26.3% （5/19）, 35.3% （6/17）, and 27.8% （5/18）, respectively; the incidences in stomach were 21.1% （4/19）, 0.0% （0/19）, 41.2% （7/17）, and 0.0% （0/18）, respectively; the incidences in kidney were 0.0% （0/19）, 0.0% （0/19）, 11.8% （2/17）, and 0.0% （0/18）, respectively. The occurrences were 68.4% （13/19）, 73.7% （14/19）, 64.7% （11/17）, and 55.6% （10/18） for liver precancerous lesions, 78.9% （15/19）, 68.4% （13/19）, 29.4% （5/17）, and 27.8% （5/18） for stomach precancerous lesions, and 42.1% （8/19）, 47.4% （9/19）, 58.8% （10/17）, and 33.3% （6/18） for kidney precancerous lesions, respectively. The occurrences of precancerous lesions were all significantly higher than those of the control mice （P〈 0.05）; the occurrence of stomach cancer and precancerous lesions of the benzo[a]pyrene 20mg/kg group were higher compared with these of the benzo[a]pyrene lOmg/kg group （P〈0.05）. No obvious lesions were found in the lung sampls. The serum IL-2 and IL-6 levels in the experiment groups were lower than those in the controls （P〈 0.01）.[ Conclusion ] Anthracene, benzo[a]pyrene, and benzo[g, h, ]perylene induce cancers and precancerous lesions in the liver, stomach, and kidney of mice, but not in the lung, indicating various target organs of polycyclic aromatic hydrocarbons. According to the reduced serum IL-2 and IL-6 levels in the experiment groups compared with the control group, we suggest that polycyclic aromatic hydrocarbons present significant inhibition to immune functions and assumed that carcinogenicity of benzo[g, h, i]perylene is strongest, followed by benzo[a]pyrene, and anthracene is lowest.