文献类型：期刊文献

英文题名：Trajectory Analysis Unveils Reelin's Role in the Directed Migration of Granule Cells in the Dentate Gyrus

作者：Wang, Shaobo[1,2];Brunne, Bianka[1];Zhao, Shanting[1,3];Chai, Xuejun[1];Li, Jiawei[1,7];Lau, Jeremie[1];Failla, Antonio Virgilio[4];Zobiak, Bernd[4];Sibbe, Mirjam[5];Westbrook, Gary L.[6];Lutz, David[1];Frotscher, Michael[1]

第一作者：Wang, Shaobo

通信作者：Lutz, D[1]

机构：[1]Ctr Mol Neurobiol ZMNH, Inst Struct Neurobiol, D-20251 Hamburg, Germany;[2]Univ Freiburg, Fac Biol, D-79104 Freiburg, Germany;[3]Northwest A&F Univ, Coll Vet Med, Yanglin 712100, Peoples R China;[4]Univ Med Ctr Hamburg Eppendorf, UKE Microscopy Imaging Facil, D-20246 Hamburg, Germany;[5]Univ Freiburg, Inst Anat & Cell Biol, D-79104 Freiburg, Germany;[6]Oregon Hlth & Sci Univ, Vollum Inst, Portland, OR 97239 USA;[7]Gansu Univ Chinese Med, Coll Basic Med, Lanzhou 730000, Gansu, Peoples R China

第一机构：Ctr Mol Neurobiol ZMNH, Inst Struct Neurobiol, D-20251 Hamburg, Germany

通信机构：[1]corresponding author), Univ Med Ctr Hamburg Eppendorf, Inst Struct Neurobiol, Ctr Mol Neurobiol Hamburg, Falkenried 94, D-20251 Hamburg, Germany.

年份：2018

卷号：38

期号：1

起止页码：137

外文期刊名：JOURNAL OF NEUROSCIENCE

收录：;Scopus(收录号：2-s2.0-85040033684);WOS:【SCI-EXPANDED(收录号:WOS:000419734200013)】；

基金：This work was supported by Deutsche Forschungsgemeinschaft Grants FR 620/12-2 and FR 620/13-1 to M.F. and Grant BR4888/2-1 to B.B., the Hertie Foundation to M.F., and the China Scholarship Council to S.W. D.L. was supported by Medical Faculty, University Medical Center Hamburg-Eppendorf FFM Fellowship. We thank Dr. Joachim Herz for providing reeler mice and mutants deficient in Reelin receptors and Dab1, respectively; Dr. Irm Hermans-Borgmeyer for embryo transfer; Bettina Herde, Dagmar Drexler, Janice Graw, Dung Ludwig, and Saskia Siegel for genotyping; and Silvana Deutsch and Kristian Schacht for animal care.

语种：英文

外文关键词：Dab1 phosphorylation; hippocampus; live imaging; neuronal migration; reeler-like; Reelin

摘要：Reelin controls neuronal migration and layer formation. Previous studies in reeler mice deficient in Reelin focused on the result of the developmental process in fixed tissue sections. It has remained unclear whether Reelin affects the migratory process, migration directionality, or migrating neurons guided by the radial glial scaffold. Moreover, Reelin has been regarded as an attractive signal because newly generated neurons migrate toward the Reelin-containing marginal zone. Conversely, Reelin might be a stop signal because migrating neurons in reeler, but not in wild-type mice, invade the marginal zone. Here, we monitored the migration of newly generated proopiomelanocortin-EGFP-expressing dentate granule cells in slice cultures from reeler, reeler-like mutants and wild-type mice of either sex using real-time microscopy. We discovered that not the actual migratory process and migratory speed, but migration directionality of the granule cells is controlled by Reelin. While wild-type granule cells migrated toward the marginal zone of the dentate gyrus, neurons in cultures from reeler and reeler-like mutants migrated randomly in all directions as revealed by vector analyses of migratory trajectories. Moreover, live imaging of granule cells in reeler slices cocultured to wild-type dentate gyrus showed that the reeler neurons changed their directions and migrated toward the Reelin-containing marginal zone of the wild-type culture, thus forming a compact granule cell layer. In contrast, directed migration was not observed when Reelin was ubiquitously present in the medium of reeler slices. These results indicate that topographically administered Reelin controls the formation of a granule cell layer.