文献类型：期刊文献

英文题名：ELL associated factor 2 is a potential diagnostic and prognostic indicator: evidence from the in silico and in vitro experiments

作者：Yu, Xiao[1];Teng, Jing-Quan[1];Chen, Jia[1];Peng, Chao[1];Guo, Tong-Rui[2];Dong, Meng-Wei[2];Hao, Xiao-Lu[1];Li, Kai-Xin[1];Huo, Xiang-Xiang[3];Ren, Cui-Cui[4]

第一作者：于霞

通信作者：Peng, C[1]

机构：[1]Gansu Univ Chinese Med, Lanzhou, Gansu, Peoples R China;[2]Hebei Prov Hosp Tradit Chinese Med, Shijiazhuang, Hebei, Peoples R China;[3]Shaanxi Univ Chinese Med, Xianyang, Shaanxi, Peoples R China;[4]Xian First Hosp, Xian, Shaanxi, Peoples R China

第一机构：甘肃中医药大学

通信机构：[1]corresponding author), Gansu Univ Chinese Med, Lanzhou, Gansu, Peoples R China.|[10735]甘肃中医药大学;

年份：2023

卷号：13

期号：6

起止页码：2572

外文期刊名：AMERICAN JOURNAL OF CANCER RESEARCH

收录：;WOS:【SCI-EXPANDED(收录号:WOS:001024486600024)】；

语种：英文

外文关键词：Neoplasm; biomarkers; metastasis

摘要：Due to the lack of sensitive biomarkers, cancer disease kill 9.6 million individuals each year around the globe. The present study aimed to explore the association between ELL Associated Factor 2 (EAF2) expression and its diagnostic and prognostic landscape across different human cancers using an in silico and in vitro approach. To achieve the defined goals of this study, we used the following online sources: UALCAN, KM plotter, TNMplot, cBioPortal, STRING, DAVID, MuTarget, Cytoscape, and CTD. In addition to this, we also used additional The Cancer Genome Atlas (TCGA) datasets via TIMER2, GENT2, and GEPIA to confirm the expression of EAF2 on additional cohorts. Finally, we performed RNA sequencing (RNA-seq) and targeted bisulfite sequencing (bisulfite-seq) techniques-based analysis using A549, ABC-1, EBC-1, LK-2 lung cancer cell lines, and MRC-9 normal control lung cell line for further validation of the results. On balance, EAF2 was elevated in 19 types of human cancers and its up-regulation was significantly correlated with shorter overall survival (OS), relapse-free survival (RFS), and metastasis in Liver Hepatocellular Carcinoma (LIHC) and Lung Squamous Cell Carcinoma (LUSC) patients. We further evaluated that EAF2 expression was also elevated across LIHC and LUSC patients belonging to different clinicopathological features. Through pathway analysis, EAF2 associations were observed with four important pathways. Moreover, some worth noticing correlations were also documented between EAF2 expression and its promoter methylation level, genetic alterations, other mutant genes, tumor purity, and different immune cells infiltration. The higher EAF2 expression contributes significantly to the tumorigenesis and metastasis of LIHC and LUSC. Therefore, it can be used as a common biomarker in these cancers.