文献类型：期刊文献

中文题名：基于UPLC-Q-TOF/MS的当归化学成分鉴定及活血作用机制

英文题名：Identification of chemical components of Angelica sinensis using UPLC-Q-TOF/MS and its the effect and mechanism of activating blood circulation

作者：焦文星[1,2,3];魏江霞[2];郭晶晶[2];杨志军[2,4,5];杨锡仓[1,3];杨秀娟[2,4,5]

第一作者：焦文星

机构：[1]甘肃中医药大学附属医院药学部,甘肃兰州730000;[2]甘肃中医药大学药学院,甘肃兰州730000;[3]杨锡仓全国名老中医药专家传承工作室,甘肃兰州730000;[4]西北中藏药省部共建协同创新中心,甘肃兰州730000;[5]陇药产业创新研究院,甘肃兰州730000

第一机构：甘肃中医药大学第二附属医院

年份：2025

卷号：41

期号：1

起止页码：147

中文期刊名：中国药理学通报

外文期刊名：Chinese Pharmacological Bulletin

收录：CSTPCD;;Scopus;北大核心:【北大核心2023】；

基金：国家自然科学基金资助项目(82160755);杨锡仓全国名老中医药专家传承工作室建设项目(国中医药人教函[2022]75号):甘肃省教育厅产业支撑计划项目(2021CYZC-21):甘肃省教育厅项目(2021B-157);甘肃省中医药管理局(GZKP-2022-37);西北中藏药协同创新中心开放基金(Xbzzy-2022-08):甘肃省中药炮制技术传承基地建设揭榜挂帅项目(2022-15)。

语种：中文

中文关键词：当归;化学成分;蛋白质组学;急性血瘀证;活血作用;作用机制

外文关键词：Angelica sinensis;chemical components;proteomics;acute blood stasis syndrome;effect of activating blood circulation;mechanism of action

摘要：目的研究当归的化学成分及其活血作用机制。方法UPLC-Q-TOF-MS鉴定化学成分;观察大鼠证侯学和心脏病理切片变化;检测血液动力学指标和蛋白质组学。结果当归共鉴定出270个化合物;给药后,大鼠弓背、耸毛,蜷缩少动,爪尾部紫暗,耳缘及鼻唇发白,饮水、进食量减少,对外界刺激反应迟钝等证候有很大改善;心肌纤维排列轻度紊乱,较模型组紧密,心肌纤维较窄,接近正常,周围可见轻度水肿、渗出,炎性细胞浸润;颈总动脉收缩压明显降低、左心室收缩压明显升高(P<0.05)。共筛选出62个差异蛋白,GO功能集中在细胞外基质、细胞骨架蛋白结合和蛋白水解的负调节等,KEGG通路富集在补体和凝血级联反应、白细胞经内皮细胞迁移及趋化因子信号通路等。结论当归可能是通过蛋白的表达,调控补体和凝血级联反应及血管平滑肌收缩等信号通路达到补血的效果。
Aim To identify the chemical components of Angelica sinensis(AS)and explore the mechanism of AS in activating blood circulation.Methods UPLC-Q-TOF-MS was used to identify the chemical components of AS.The changes of syndrome and pathological section of heart in rats were observed.Hemodynamics and proteomics were measured.Results A total of 270 compounds were identified from AS.It showed that rats of Angelica sinensis group were greatly improved such as arched back,shrugged fur,huddled up and less mobile,purplish paws and tails,whitish ear margins and nasolabial lips,reduced drinking and feeding,and slow response to external stimuli;mildly disordered myocardial fibre arrangement,myofibre arrangement was tighter than that of model group,myocardial fibres were narrower and close to normal,and mild oedema,exudation,and inflammatory cell infiltration could be seen in the surrounding area;SAP was significantly lower and LVSP was significantly higher in Angelica sinensis group(P<0.05).Proteomics showed that 62 differential proteins were screened in Angelica sinensis group compared to model,GO function were concentrated in the extracellular matrix,cytoskeletal proteins binding and protein hydrolysis negatively regulated.KEGG pathway were enriched in signalling pathways such as complement and coagulation cascades,cellular focal adhesion,leukocyte transendothelial migration and chemokine signalling pathways.Conclusions AS probably through the expression of proteins,which modulate the signalling pathways of the complement and coagulation cascade reactions and the contraction of vascular smooth muscle.